Flow Cytometry :First Principles

Publication subTitle :First Principles

Author: Alice Longobardi Givan  

Publisher: John Wiley & Sons Inc‎

Publication year: 2004

E-ISBN: 9780471459118

P-ISBN(Paperback): 9780471382249

P-ISBN(Hardback):  9780471382249

Subject: Q2-33 experimental cytology and cytology method

Language: ENG

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Description

Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis.

Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource:

  • Assumes no prior knowledge of flow cytometry
  • Progresses with an informal, engaging lecture style from simpleto more complex concepts
  • Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis
  • Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields

Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

Chapter

Contents

pp.:  1 – 8

Preface

pp.:  8 – 12

Acknowledgments (First Edition)

pp.:  12 – 16

1 The Past as Prologue

pp.:  18 – 20

2 Setting the Scene

pp.:  20 – 30

3 Instrumentation: Into the Black Box

pp.:  30 – 34

4 Information: Harnessing the Data

pp.:  34 – 60

5 Seeing the Light: Lasers, Fluorochromes, and Filters

pp.:  60 – 78

6 Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating

pp.:  78 – 100

7 Cells from Within: Intracellular Proteins

pp.:  100 – 134

8 Cells from Within: DNA in Life and Death

pp.:  134 – 142

9 The Sorting of Cells

pp.:  142 – 178

10 Disease and Diagnosis: The Clinical Laboratory

pp.:  178 – 194

11 Out of the Mainstream: Research Frontiers

pp.:  194 – 214

12 Flowing On: The Future

pp.:  214 – 244

General References

pp.:  244 – 248

Glossary

pp.:  248 – 254

Figure Credits

pp.:  254 – 276

Index

pp.:  276 – 282

LastPages

pp.:  282 – 293

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