Some Biological Techniques in Electron Microscopy

Author: Parsons   D. F.  

Publisher: Elsevier Science‎

Publication year: 2012

E-ISBN: 9780323160353

P-ISBN(Paperback): 9780125455503

P-ISBN(Hardback):  9780125455503

Subject: Q938 microbial ecology and regional distribution

Language: ENG

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Description

Some Biological Techniques in Electron Microscopy discusses the problems that hinder or prevent progress in biological electron microscopy. This book explores the great potential of electron microscope for solving several pressing medical and biological research problems.
Organized into five chapters, this book starts with an overview of the primary goal of biological electron microscopy, which is the visualization of atoms in biological molecules and structures. This text proceeds with a discussion of the freezing methods of electron microscopy in which some of the artifacts producing steps of environmental techniques are eliminated. Other chapters consider the objective of electron microscopy to resolve successively small objects, in the limiting instance, single atoms in amorphous structures. The final chapter deals with processing tissues for electron microscopy wherein temperature, agitation, pressure, and humidity can be controlled throughout the operation.
Biologists, biophysicists, physicists, electron microscopists, and medical researchers will find this book extremely useful.

Chapter

Front Cover

pp.:  1 – 4

Copyright Page

pp.:  5 – 6

Table of Contents

pp.:  6 – 8

List of Contributors

pp.:  8 – 10

Preface

pp.:  10 – 14

Chapter I. Problems in High Resolution Electron Microscopy of Biological Materials in Their Natural State

pp.:  14 – 82

Chapter II. Chemical Effects of Fixation on Biological Specimens

pp.:  82 – 114

Chapter III. Present Status of Freezing Techniques

pp.:  114 – 160

Chapter IV. Reducing the Effect of Substrate Noise in Electron Images of Biological Objects

pp.:  160 – 178

Chapter V. Automation in Tissue Processing for Electron Microscopy

pp.:  178 – 188

Author Index

pp.:  188 – 196

Subject Index

pp.:  196 – 200

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