Description
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
RNA Interference will cover RNAi in non-vertebrates (plants, C. elegans, drosophila, and S. pombe), and Mammalian systems (human and non-human cells). This volume discusses extensive methodology related to delivery methods high throughput strategies and prospects as a human therapy agent.
* One of the most highly respected publications in the field of biochemistry since 1955
* Frequently consulted, and praised by researchers and reviewers alike
* Truly an essential publication for anyone in any field of the life sciences
Chapter
[1] Transgene-Induced RNA Interference as a Tool for Plant Functional Genomics
pp.：
37 – 60
[2] Constructs and Methods for Hairpin RNA-Mediated Gene Silencing in Plants
pp.：
60 – 72
[3] RNA Interference in Caenorhabditis elegans
pp.：
72 – 91
[4] High-Throughput RNA Interference Screens in Drosophila Tissue Culture Cells
pp.：
91 – 109
[5] Mechanistic Insights Aid Computational Short Interfering RNA Design
pp.：
109 – 133
[6] Novel Methods for Expressing RNA Interference in Human Cells
pp.：
133 – 148
[7] Delivery of Small Interfering RNA to Mammalian Cells in Culture by Using Cationic Lipid/Polymer-Based Transfection Reagents
pp.：
148 – 161
[8] Subcellular Distribution of Small Interfering RNA: Directed Delivery Through RNA Polymerase III Expression Cassettes and Localization by In Situ Hybridization
pp.：
161 – 181
[9] Viral Delivery of Recombinant Short Hairpin RNAs
pp.：
181 – 209
[10] Targeting Cellular Genes with PCR Cassettes Expressing Short Interfering RNAs
pp.：
209 – 222
[11] Use of Short Hairpin RNA Expression Vectors to Study Mammalian Neural Development
pp.：
222 – 235
[12] Analysis of T-Cell Development by Using Short Interfering RNA to Knock Down Protein Expression
pp.：
235 – 254
[13] Lentiviral Vector Delivery of Recombinant Small Interfering RNA Expression Cassettes
pp.：
254 – 263
[14] Paradigms for Conditional Expression of RNA Interference Molecules for Use Against Viral Targets
pp.：
263 – 278
[15] High-Throughput RNA Interference Strategies for Target Discovery and Validation by Using Synthetic Short Interfering RNAs: Functional Genomics Investigations of Biological Pathways
pp.：
278 – 314
[16] RNA Interference In Vivo: Toward Synthetic Small Inhibitory RNA-Based Therapeutics
pp.：
314 – 333
[17] Labeling and Characterization of Small RNAs Associated with the RNA Interference Effector Complex RITS
pp.：
333 – 344
[18] RNA Interference Spreading in C. elegans
pp.：
344 – 352
[19] Human Dicer: Purification, Properties, and Interaction with PAZ PIWI Domain Proteins
pp.：
352 – 372
[20] Delivery of siRNA and siRNA Expression Constructs to Adult Mammals by Hydrodynamic Intravascular Injection
pp.：
372 – 387
[21] Analysis of Short Interfering RNA Function in RNA Interference by Using Drosophila Embryo Extracts and Schneider Cells
pp.：
387 – 407
[22] Use of RNA Polymerase II to Transcribe Artificial MicroRNAs
pp.：
407 – 417
[23] Adeno-Associated Virus Vectors for Short Hairpin RNA Expression
pp.：
417 – 441
[24] Simple, Robust Strategies for Generating DNA-Directed RNA Interference Constructs
pp.：
441 – 456
Author Index
pp.：
456 – 479
Subject Index
pp.：
479 – 488
RNA Interference
( Volume 392 )
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