Description
On the cutting edge of medical diagnostics, plasma proteomics promises to generate a new wave of technologies to help identify many different diseases and disease risks.
Plasma and serum are the preferred non-invasive specimens to test normal individuals, at-risk groups, and patients for protein biomarkers discovered and validated to reflect physiological, pathological, and pharmacological phenotypes. These specimens present enormous challenges due to extreme complexity, huge dynamic range in protein concentrations, non-standardized methods of sample processing, and intra- and inter-individual variation from genetics, diet, smoking, hormones, and other sources. This book presents the major findings from the collaborative Plasma Proteome Project organized by the international Human Proteome Organization (HUPO). The chapters are drawn from a larger set of publications in the journal PROTEOMICS. This book provides a valuable foundation for development and applications of proteomics.
Chapter
3 HUPO Plasma Proteome Project specimen collection and handling: Towards the standardization of parameters for plasma proteome samples
pp.：
61 – 87
4 Immunoassay and antibody microarray analysis of the HUPO Plasma Proteome Project reference specimens: Systematic variation between sample types and calibration of mass spectrometry data
pp.：
87 – 115
5 Depletion of multiple high-abundance proteins improves protein profiling capacities of human serum and plasma
pp.：
115 – 139
6 A novel four-dimensional strategy combining protein and peptide separation methods enables detection of low-abundance proteins in human plasma and serum proteomes
pp.：
139 – 159
7 A study of glycoproteins in human serum and plasma reference standards (HUPO) using multilectin affinity chromatography coupled with RPLC-MS/MS
pp.：
159 – 183
8 Evaluation of prefractionation methods as a preparatory step for multidimensional based chromatography of serum proteins
pp.：
183 – 209
9 Efficient prefractionation of low-abundance proteins in human plasma and construction of a two-dimensional map
pp.：
209 – 225
10 Comparison of alternative analytical techniques for the characterization of the human serum proteome in HUPO Plasma Proteome Project
pp.：
225 – 245
11 A proteomic study of the HUPO Plasma Proteome Project’s pilot samples using an accurate mass and time tag strategy
pp.：
245 – 273
12 Analysis of Human Proteome Organization Plasma Proteome Project (HUPO PPP) reference specimens using surface enhanced laser desorption/ionization-time of flight (SELDI-TOF) mass spectrometry: Multi-institution correlation of spectra and identification of biomarkers
pp.：
273 – 297
13 An evaluation, comparison, and accurate benchmarking of several publicly available MS/MS search algorithms: Sensitivity and specificity analysis
pp.：
297 – 313
14 Human Plasma PeptideAtlas
pp.：
313 – 341
15 Do we want our data raw? Including binary mass spectrometry data in public proteomics data repositories
pp.：
341 – 347
16 A functional annotation of subproteomes in human plasma
pp.：
347 – 353
17 Cardiovascular-related proteins identified in human plasma by the HUPO Plasma Proteome Project Pilot Phase
pp.：
353 – 377
Exploring the Human Plasma Proteome
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
