Introduction to Modern Liquid Chromatography

Author： Lloyd R. Snyder  

Publisher： John Wiley & Sons Inc‎

Publication year： 2009

E-ISBN: 9780470508176

P-ISBN(Hardback):  9780470167540

Subject： O657.7 chromatographic analysis

Language： ENG

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Description

The latest edition of the authoritative reference to HPLC

High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples.

This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as:

  • The basis of HPLC separation and the general effects of different experimental conditions
  • Equipment and detection
  • The column—the "heart" of the HPLC system
  • Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques
  • Computer simulation, qualitative and quantitative analysis, and method validation and quality control
  • The separation of large molecules, including both biological and synthetic polymers
  • Chiral separations, preparative separations, and sample preparation
  • Systematic development of HPLC separations—new to this edition
  • Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms
Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Chapter

CONTENTS

pp.：  7 – 33

PREFACE

pp.：  33 – 37

GLOSSARY OF SYMBOLS AND ABBREVIATIONS

pp.：  37 – 45

1 INTRODUCTION

pp.：  45 – 63

2 BASIC CONCEPTS AND THE CONTROL OF SEPARATION

pp.：  63 – 131

3 EQUIPMENT

pp.：  131 – 191

4 DETECTION

pp.：  191 – 243

5 THE COLUMN

pp.：  243 – 297

6 REVERSED-PHASE CHROMATOGRAPHY FOR NEUTRAL SAMPLES

pp.：  297 – 347

7 IONIC SAMPLES: REVERSED-PHASE, ION-PAIR, AND IONEXCHANGE CHROMATOGRAPHY

pp.：  347 – 405

8 NORMAL-PHASE CHROMATOGRAPHY

pp.：  405 – 447

9 GRADIENT ELUTION

pp.：  447 – 519

10 COMPUTER-ASSISTED METHOD DEVELOPMENT

pp.：  519 – 543

11 QUALITATIVE AND QUANTITATIVE ANALYSIS

pp.：  543 – 575

12 METHOD VALIDATION

pp.：  575 – 613

13 BIOCHEMICAL AND SYNTHETIC POLYMER SEPARATIONS

pp.：  613 – 709

14 ENANTIOMER SEPARATIONS

pp.：  709 – 769

15 PREPARATIVE SEPARATIONS

pp.：  769 – 801

16 SAMPLE PREPARATION

pp.：  801 – 853

17 TROUBLESHOOTING

pp.：  853 – 923

APPENDIX I. PROPERTIES OF HPLC SOLVENTS

pp.：  923 – 931

APPENDIX II. PREPARING BUFFERED MOBILE PHASES

pp.：  931 – 935

Index

pp.：  935 – 956

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