RNA Interference in Practice
：Principles, Basics, and Methods for Gene Silencing in C. elegans, Drosophila, and Mammals
Publication subTitle ：Principles, Basics, and Methods for Gene Silencing in C. elegans, Drosophila, and Mammals
Author： Ute Schepers
Publisher： John Wiley & Sons Inc
Publication year： 2006
E-ISBN: 9783527604371
P-ISBN(Hardback): 9783527310203
Subject： Q522 ribose (deoxyribose nucleic acid (RNA)
Language： ENG
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Description
This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids.
In easy-to-follow, step-by-step protocols you will learn
* how RNAi works in worms, flies and mammals,
* how to design the most efficient RNAi constructs,
* how to achieve transient, stable and conditional RNAi in cell cultures,
* how to determine the efficiency of an RNAi experiment,
* and how to use RNAi for gene therapy.
All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.
Chapter