Route Maps in Gene Technology

Author: Mark Walker  

Publisher: John Wiley & Sons Inc‎

Publication year: 2009

E-ISBN: 9781444313604

P-ISBN(Paperback): 9780632037926

P-ISBN(Hardback):  9780632037926

Subject: Q78 genetic engineering (genetic engineering)

Language: ENG

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Description

Route Maps in Gene Technology is an exciting new introductory textbook for first-year undergraduates in molecular biology and molecular genetics. The subject is broken down into 140 to 150 key concepts or topics, each of which is dealt with in one doublepaged spread. These range from basic introductory principles to applied topics at the cutting edge of research. A control strip along the top of the page shows the student which pages need to have been read beforehand and which topics may be followed afterward. In addition, at the front of the book are a selection of 'routes,' which the student or teacher may choose in order to study a particular topic. Because courses have become more 'modular' and many students arrive at college with little or no biology background, this approach enables teachers and students to structure a course of study to best suit their disparate exposure to biology.

  • An exciting new concept in textbook design, allowing unparalleled flexibility on the part of the student and the teacher
  • Covers the full range of modern molecular biology, from basic principles to the latest applications
  • Attractive, clear and simple presentation with copious two-colour illustrations

Chapter

CONTENTS

pp.:  7 – 9

PREFACE

pp.:  9 – 11

ABOUT THE ROUTE MAPS FORMAT

pp.:  11 – 12

3. GENES ARE COMPOSED OF DNA

pp.:  16 – 18

10. DISTRIBUTION OF NUCLEIC ACIDS WITHIN EUKARYOTIC CELLS

pp.:  32 – 34

11. TYPES OF RNA MOLECULES

pp.:  34 – 36

12. THE ANATOMY OF EUKARYOTIC CHROMOSOMES

pp.:  36 – 38

13. ORGANISATION OF GENES WITHIN CHROMOSOMES

pp.:  38 – 42

14. THE MOLECULAR ANATOMY OF EUKARYOTIC GENES

pp.:  42 – 46

15. CHROMOSOME ABERRATIONS AND LINKS TO HUMAN DISEASE

pp.:  46 – 48

16. TYPES OF MUTATIONS AND THEIR EFFECTS

pp.:  48 – 50

17. FORMS OF CHEMICALLY ALTERED DNA

pp.:  50 – 52

18. DNA REPAIR MECHANISMS

pp.:  52 – 54

19. LINKAGE ANALYSIS

pp.:  54 – 56

20. PEDIGREE ANALYSIS AND MODES OF INHERITANCE

pp.:  56 – 58

21. GENES DICTATE THE NATURE OF PROTEINS

pp.:  58 – 60

22. THE NATURE OF THE GENETIC CODE

pp.:  60 – 64

23. TRANSCRIPTION: FORMING GENETIC MESSAGES

pp.:  64 – 66

24. POST-TRANSCRIPTIONAL PROCESSING OF MESSENGER RNA

pp.:  66 – 70

25. TRANSFER AND RIBOSOMAL RNA PROCESSING/MODIFICATION

pp.:  70 – 72

26. MECHANISMS REGULATING GENE EXPRESSION

pp.:  72 – 74

27. TRANSCRIPTIONAL REGULATORY SEQUENCES

pp.:  74 – 76

28. OPERONS AND PROKARYOTIC CONTROL OF GENE EXPRESSION

pp.:  76 – 78

29. TRANSCRIPTION FACTORS AND GENE EXPRESSION

pp.:  78 – 80

30. IN VIVO TRANSLATION: DECODING GENETIC MESSAGES

pp.:  80 – 84

31. SEQUENCES INVOLVED IN CELLULAR PROTEIN TARGETING

pp.:  84 – 86

32. EUKARYOTIC CELL DIVISION: MITOSIS AND MEIOSIS

pp.:  86 – 90

33. MOLECULAR MECHANISMS OF CELL CYCLE CONTR0L

pp.:  90 – 92

34. GENETIC RECOMBINATION MECHANISMS

pp.:  92 – 94

35. GENE TRANSFER DURING BACTERIAL REPRODUCTION

pp.:  94 – 96

36. TRANSPOSABLE GENETIC ELEMENTS: TRANSPOSONS

pp.:  96 – 100

37. IN VIVO DNA REPLICATION

pp.:  100 – 104

38. GENETIC CONTROL OF DEVELOPMENT

pp.:  104 – 108

39. THE NATURAL BIOLOGY OF BACTERIOPHAGES

pp.:  108 – 110

40. BACTERIOPHAGE GENETICS

pp.:  110 – 114

41. RECOMBINANT DNA TECHNOLOGY

pp.:  114 – 116

42. ENZYMES COMMONLY USED IN MOLECULAR BIOLOGY METHODS

pp.:  116 – 118

43. RESTRICTION ENDONUCLEASES

pp.:  118 – 120

44. RESTRICTION FRAGMENT LENGTH POLYMORPHISMS

pp.:  120 – 122

45. ISOLATION OF NUCLEIC ACIDS FROM CELLS AND TISSUES

pp.:  122 – 126

46. VISUALISING NUCLEIC ACIDS

pp.:  126 – 128

47. ELECTROPHORESIS OF NUCLEIC ACIDS

pp.:  128 – 130

48. IN VITRO HYBRIDISATION

pp.:  130 – 132

49. TYPES OF HYBRIDISATION ASSAY FORMATS

pp.:  132 – 134

50. SOUTHERN BLOTTING

pp.:  134 – 136

51. IN SITU HYBRIDISATION

pp.:  136 – 138

52. MEASURING TRANSCRIPTIONAL ACTIVITY VIA MESSENGER RNA

pp.:  138 – 140

53. CONVERTING MESSENGER RNA INTO COMPLEMENTARY DNA

pp.:  140 – 142

54. METHODS FOR DETERMINING DNA NUCLEOTIDE SEQUENCES

pp.:  142 – 146

55. THE POLYMERASE CHAIN REACTION

pp.:  146 – 150

56. ALTERNATIVES TO PCR-BASED IN VITRO DNA/RNA AMPLIFICATION

pp.:  150 – 154

57. IN VITRO TRANSLATION METHODS

pp.:  154 – 156

58. TYPES AND METHODS OF GENE PROBE GENERATION

pp.:  156 – 158

59. CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES

pp.:  158 – 162

60. TYPES AND APPLICATIONS OF NUCLEOTIDE ANALOGUES

pp.:  162 – 164

61. METHODS FOR LABELLING GENE PROBES

pp.:  164 – 168

62. FUNDAMENTAL PRINCIPLES OF CLONING

pp.:  168 – 170

63. THE NATURE OF CLONING VECTORS

pp.:  170 – 172

64. INSERTING FOREIGN DNA INTO VECTORS

pp.:  172 – 174

65. THE DEVELOPMENT OF BACTERIOPHAGE VECTORS

pp.:  174 – 178

66. PLASMIDS: DEVELOPMENT AS CLONING VECTORS

pp.:  178 – 182

67. YEAST-DERIVED PLASMID VECTORS

pp.:  182 – 184

68. PHAGEMIDS, HYBRID PHAGE AND PLASMID CLONING VECTORS

pp.:  184 – 186

69. VECTORS FOR USE IN PLANT AND ANIMAL CELLS

pp.:  186 – 188

70. DELIVERING DNA INTO CELLS

pp.:  188 – 190

71. PRINCIPAL GENOMIC AND cDNA CLONING STRATEGIES

pp.:  190 – 194

72. STRATEGIES FOR IDENTIFYING DESIRABLE RECOMBINANT CLONES

pp.:  194 – 196

73. GENE MAPPING TECHNIQUES

pp.:  196 – 200

74. DETECTING DNA-PROTEIN INTERACTION SITES

pp.:  200 – 204

75. DETECTING PROMOTER AND ENHANCER SEQUENGES

pp.:  204 – 206

76. METHODS FOR IDENTIFYING PROTEIN-ENCODING REGIONS

pp.:  206 – 210

77. GENETIC FINGERPRINTING

pp.:  210 – 212

78. ANALYSING ANCIENT DNA

pp.:  212 – 214

79. IN VITRO MUTAGENESIS METHODS

pp.:  214 – 218

80. GENETICALLY MODIFIED MICRO-ORGANISMS

pp.:  218 – 220

81. GENETICALLY ENGINEERED PLANTS

pp.:  220 – 222

82. GENETICALLY ENGINEERED ANIMALS

pp.:  222 – 224

83. MOLECULAR TECHNIQUES IN PRENATAL DIAGNOSIS

pp.:  224 – 226

84. THE GENETICS OF CYSTIC FIBROSIS

pp.:  226 – 230

85. THE DYSTROPHIN GENE AND MUSCULAR DYSTROPHIES

pp.:  230 – 234

86. IDENTIFYING THE GENE FOR HUNTINGTON'S DISEASE

pp.:  234 – 236

87. LIPOPROTEIN GENES AND CORONARY HEART DISEASE

pp.:  236 – 238

88. THE DETECTION OF MICROBIAL INFECTIONS

pp.:  238 – 240

89. MOLECULAR BIOLOGY OF HUMAN IMMUNODEFICIENCY VIRUS AND AIDS

pp.:  240 – 244

90. ENGINEERING MICROBIAL BIOLUMINESCENCE

pp.:  244 – 246

91. RECOMBINANT DNA METHODS IN VACCINE DEVELOPMENT

pp.:  246 – 248

92. RECEPTORS AND CELLULAR SIGNALLING MECHANISMS

pp.:  248 – 252

93. ONCOGENES AND THE MOLECULAR BASIS OF CANCER

pp.:  252 – 256

94. MOLECULAR DIAGNOSIS AND THERAPY OF CANCERS

pp.:  256 – 258

95. DRUG DEVELOPMENT USING RECOMBINANT DNA APPROACHES

pp.:  258 – 260

96. PROTEIN ENGINEERING

pp.:  260 – 264

97. IMMUNOGLOBULIN GENETICS

pp.:  264 – 268

98. GENETIC ENGINEERING OF RECOMBINANT ANTIBODIES

pp.:  268 – 272

99. CURRENT APPROACHES TO GENE THERAPY

pp.:  272 – 274

100. THE HUMAN GENOME MAPPING PROJECT

pp.:  274 – 278

BIBLIOGRAPHY

pp.:  278 – 323

INDEX

pp.:  323 – 336

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