Andrology. Reduction of steps in the preparation of motile sperm for intrauterine insemination does not reduce efficacy of the procedure: simplified one-step swim-up method versus classic swim-up

Author： Inaudi P. Petrilli S. Joghtapour A. Trusso P.

Publisher： Oxford University Press

ISSN： 1460-2350

Source： Human Reproduction, Vol.17, Iss.5, 2002-05, pp. : 1288-1291

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Abstract

BACKGROUND: Intrauterine insemination (IUI) is a valid treatment for infertility with a cumulative pregnancy rate of >40–90% after 3–10 treatment cycles. We studied the efficacy of a simplified method for motile sperm preparation for IUI. METHODS: A prospective clinical trial was performed with 100 couples (male age 33–48 and female 28–37 years) with a 2–8 year history of primary infertility associated with slight oligozoospermia (16/100), oligomenorrhoea (32/100) or unknown (52/100). Motile sperm for IUI were prepared by: (A) the classic World Health Organization self-migration (swim-up) method which includes centrifugation, or (B) a simplified one-step swim-up procedure without centrifugation. Recombinant FSH was used for ovarian stimulation. Depending on the cause of infertility, patients were matched one-to-one at the time of IUI, so that when a total of 100 couples had been treated, 50/100 women received sperm prepared by method A and 50/100 by method B. RESULTS: A statistically significant correlation was found between the percentage motile sperm of the original semen sample and the percentage of motile sperm recovered by method A (r = 0.333, P < 0.01)="" and="" b="">r = 0.400, P < 0.01).="" a="" highly="" significant="" correlation="">r = 0.997, P < 0.001)="" was="" found="" between="" the="" two="" methods.="" conclusions:="" the="" simplified="" one-step="" swim-up="" method="" was="" as="" effective="" as="" the="" classic="" swim-up="" method,="" but="" the="" former="" was="" easier="" and="" more="" economical.="">