Single Molecule Dynamics in Life Science

Author： Toshio Yanagida  

Publisher： John Wiley & Sons Inc‎

Publication year： 2008

E-ISBN: 9783527626144

P-ISBN(Hardback):  9783527312887

Subject： Q7 Molecular Biology

Language： ENG

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Description

In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment.
Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.

Chapter

Contents

pp.：  1 – 7

Preface

pp.：  7 – 15

List of Contributors

pp.：  15 – 17

1 A Road Map to Single Molecule Dynamics

pp.：  17 – 21

2 Single Molecule Study for Elucidating the Mechanism Used by Biosystems to Utilize Thermal Fluctuations

pp.：  21 – 31

3 Imaging and Molecular Motors

pp.：  31 – 61

4 Ion Channels

pp.：  61 – 107

5 Signal Transduction across the Plasma Membrane

pp.：  107 – 119

6 Dynamics of Membrane Receptors: Single-molecule Tracking of Quantum Dot Liganded Epidermal Growth Factor

pp.：  119 – 137

7 Studying the Dynamics of Ligand–Receptor Complexes by Single-Molecule Techniques

pp.：  137 – 151

8 RNA in cells

pp.：  151 – 191

9 Protein Dynamics and Interactions

pp.：  191 – 211

10 Two Rotary Motors of ATP Synthase

pp.：  211 – 257

11 Single-molecule FRET Studies of Helicases and Holliday Junctions

pp.：  257 – 277

12 High-speed Atomic Force Microscopy for Nano-visualization of Biomolecular Processes

pp.：  277 – 297

13 Force-clamp Spectroscopy of Single Proteins

pp.：  297 – 317

Index

pp.：  317 – 343

LastPages

pp.：  343 – 348

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