Protease production by Streptomyces sp. isolated from brazilian cerrado soil: Optimization of culture medium employing statistical experimental design

Author： Azeredo Luciana   Castilho Leda   Leite Selma   Coelho Rosalie   Freire Denise  

Publisher： Humana Press, Inc

ISSN： 0273-2289

Source： Applied Biochemistry and Biotechnology, Vol.108, Iss.1-3, 2003-03, pp. : 749-755

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Abstract

Streptomyces are important microorganisms because of their capacity to produce numerous bioactive molecules. In the present work protease production, by Streptomyces sp. 594 isolated from a Brazilian Cerrado soil, was maximized by optimizing a low-cost culture medium composition (casitone and sugarcane molasses) using statistical experimental design. The final protease activity (56 U/mL) was 2.8-fold and 58-fold higher than that obtained in the beginning of this study, and in a previous work, using an actinomycete selection medium, respectively. Protease production, not growth associated, appeared to be modulated by an inducer system, whereby the C/N ratio seemed to play a significant role.

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