Author： Zhang L. Hernan R. Brizzard B.
Publisher： Humana Press, Inc
ISSN： 1073-6085
Source： Molecular Biotechnology, Vol.19, Iss.3, 2001-11, pp. : 313-321
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Epitope tagging is a valuable tool for quick detection, isolation, and analysis of protein-protein interaction, without prior knowledge of the target protein. The FLAG epitope tag, one of the most widely used tags, is an eight amino acid peptide that can be detected by anti-FLAG monoclonal antibody. In the present study, we have examined the detection sensitivity of a protein fused to three tandem FLAG epitopes by Western blot analysis, immunoprecipitation, and immunohistochemical analysis using anti-FLAG® M2 antibody. We find that the triple FLAG epitope significantly enhances the sensitivity of detection of fusion protein expressed in mammalian cells.
Related content
Access to resources
Recommend
By Baldi Lucia Hacker David Adam Myriam Wurm Florian
Biotechnology Letters, Vol. 29, Iss. 5, 2007-05 ,pp. :