Author： Prado S. Díaz Tarrío N. Cerdán M. E. Siso M. I. González
Publisher： Humana Press, Inc
ISSN： 1073-6085
Source： Molecular Biotechnology, Vol.26, Iss.1, 2004-01, pp. : 35-38
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Abstract
A new polymerase chain reaction (PCR)-based method is described for the isolation of clones of interest from a library when only part of a sequence is available. In actuality, this occurs with many genomes that have been partially sequenced using a random strategy. The method presented here, discriminating clusters by PCR (DCbyPCR), is a nonradioactive and improved alternative to colony hybridization.
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