Generation of linear expression constructs by one-step PCR with vaccinia DNA topoisomerase I

Author： Xiao Jin-Hua   Xin Wen   Liu Yong-Jie   Murphy Robert   Huang Da-Wei  

Publisher： Humana Press, Inc

ISSN： 1073-6085

Source： Molecular Biotechnology, Vol.35, Iss.1, 2007-02, pp. : 15-22

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

Related content

A universal cloning vector using vaccinia topoisomerase I

By Geng Liang   Xin Wen   Huang Da-Wei   Feng Gui  

Molecular Biotechnology, Vol. 33, Iss. 1, 2006-05 ,pp. : 23-28

Humana Press, Inc

Access to resources Recommend Favorite

Expression and simple, one-step purification of fragile histidine triad (Fhit) tumor suppressor mutant forms in Escherichia coli and their interaction with protoporphyrin IX

By Zawacka-Pankau Joanna   Podhajska Anna  

Biotechnology Letters, Vol. 29, Iss. 6, 2007-06 ,pp. : 877-883

Springer Publishing Company

Access to resources Recommend Favorite

Construction of linear functional expression elements with DNA fragments created by site-specific DNA nickase, N. Bpu 10 I, and exonuclease III

By Xin Wen  

Biotechnology Letters, Vol. 25, Iss. 22, 2003-01 ,pp. : 1913-1916

Springer Publishing Company

Access to resources Recommend Favorite

Recombinant vaccinia viruses: Design, generation, and isolation

By Broder Christopher   Earl Patricia  

Molecular Biotechnology, Vol. 13, Iss. 3, 1999-12 ,pp. : 223-245

Humana Press, Inc

Access to resources Recommend Favorite

Cloning, expression, and PCR application of DNA polymerase from the hyperthermophilic archaeon, Thermococcus celer

By Kim Kee  

Biotechnology Letters, Vol. 33, Iss. 2, 2011-02 ,pp. : 339-346

Springer Publishing Company

Access to resources Recommend Favorite