Recognition and Lysis of Human Melanoma by a CD3 + , CD4 + , CD8 - T-Cell Clone Restricted by HLA-A2

Author： Darrow T.L. Abdel-Wahab Z. Quinn-Allen M.A. Seigler H.F.

ISSN： 0008-8749

Source： Cellular Immunology, Vol.172, Iss.1, 1996-08, pp. : 52-59

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Abstract

The in vitro cytotoxic response to human melanoma is characterized by CD3 + CD8 + T-cells which recognize shared peptide antigens presented in the context of HLA class-I-encoded gene products. We report here studies of a CD3 + , CD4 + , CD8 - , HLA-A2-restricted, melanoma-specific cytotoxic T-cell clone derived by limiting dilution from a T-cell line induced in PBLs from a melanoma patient following in vitro stimulation with an HLA-A2-matched melanoma cell line. The CD4 + cytotoxic T-cell clone is lytic only for melanomas which share the HLA-A2 allele, and the cytotoxicity is blocked by antibody to the T-cell receptor and by antibody to HLA class I. The clone proliferates only following stimulation with HLA-A2-matched melanoma tumor cells. The data suggest that cytotoxic CD4 + T-cells may play a significant role in immunity to melanoma, and HLA class-I-restricted recognition of melanoma may not necessarily require the CD8 molecule on the lytic T-cell.