Determination of Vesicle–Water Partition Coefficients by Electrokinetic Chromatography: Study of Temperature Effect

ISSN： 0021-9797

Source： Journal of Colloid and Interface Science, Vol.253, Iss.2, 2002-09, pp. : 397-401

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Abstract

Vesicular electrokinetic chromatography was used to investigate solute partitioning from the aqueous phase into dihexadecyl hydrogen phosphate (DHP) vesicles. Retention factors of neutral solutes are related to their partition coefficients between the aqueous phase and vesicles (K_vw). The K_vw of the aromatic test solutes were readily obtained from the slopes of the linear relationships between retention factors versus DHP concentrations. The technique offers the advantages of speed, automation, and small sample size for determination of partition coefficients. The K_vw values of 43 uncharged solutes were measured at below as well as above the phase transition temperatures. The logarithms of partition coefficients (log K_vw) of solutes at 71°C (above T_c) were slightly higher than those at 36°C (below T_c). The solvation characteristics of DHP were also studied using linear solvation energy relationships at the two temperatures. © 2002 Elsevier Science (USA).