The Linkage Change of a Knotting Reaction Catalysed by Tn3 Resolvase

Author： Stark W.M.   Boocock M.R.  

Publisher： Academic Press

ISSN： 0022-2836

Source： Journal of Molecular Biology, Vol.239, Iss.1, 1994-05, pp. : 25-36

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

Tn3 resolvase catalyses recombination between a pair of identical res sites, directly repeated on a supercoiled plasmid molecule. The normal in vitro products is a two-noded catenane. A plasmid with a single base substitution at one res site is converted to non-recombinant knots by resolvase; the first major product is a four-noded knot. The linkage change, ΔLk, on formation of the four-noded knot is +4. The formation of the observed knots from the mutant substrate, and this value of ΔLk were predicted by and in agreement with a "simple rotation" model for catalysis of strand exchange by resolvase. A number of alternative mechanisms for knotting by resolvase are inconsistent with the observed topological changes.

Related content

Interactions of Protein Complexes on Supercoiled DNA: The Mechanism of Selective Synapsis by Tn3 Resolvase

By Brown J.L.   He J.   Sherratt D.J.   Marshall Stark W.   Boocock M.R.  

Journal of Molecular Biology, Vol. 319, Iss. 2, 2002-05 ,pp. : 371-383 (13)

Academic Press

Access to resources Recommend Favorite

Topological Selectivity of a Hybrid Site-specific Recombination System with Elements from Tn3res/resolvase and Bacteriophage P1 loxP/Cre

By Kilbride E.   Boocock M.R.   Stark W.M.  

Journal of Molecular Biology, Vol. 289, Iss. 5, 1999-06 ,pp. : 1219-1230 (12)

Academic Press

Access to resources Recommend Favorite

Tn5: A Molecular Window on Transposition

By Reznikoff W.S.   Bhasin A.   Davies D.R.   Goryshin I.Y.   Mahnke L.A.   Naumann T.   Rayment I.   Steiniger-White M.   Twining S.S.  

Biochemical and Biophysical Research Communications, Vol. 266, Iss. 3, 1999-12 ,pp. : 729-734 (6)

Elsevier

Access to resources Recommend Favorite

Synapsis of Tn3 Recombination Sites: Unpaired Sites Destabilize Synapses by a Partner Exchange Mechanism

By He J.   McIlwraith M.J.   Burke M.E.   Boocock M.R.   Stark W.M.  

Journal of Molecular Biology, Vol. 319, Iss. 2, 2002-05 ,pp. : 385-393 (9)

Academic Press

Access to resources Recommend Favorite

The catalytic residues of Tn 3 resolvase

By Olorunniji Femi J.   Stark W. Marshall  

Nucleic Acids Research, Vol. 37, Iss. 22, 2009-12 ,pp. : 7590-7602 (13)

Oxford University Press

Access to resources Recommend Favorite