Publisher: Academic Press
ISSN: 0022-2836
Source: Journal of Molecular Biology, Vol.269, Iss.3, 1997-06, pp. : 342-354
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Abstract
The Res subunits of the type III restriction-modification enzymes share a statistically significant amino acid sequence similarity with several RNA and DNA helicases of the so-called DEAD family. It was postulated that in type III restriction enzymes a DNA helicase activity may be required for local unwinding at the cleavage site. The members of this family share seven conserved motifs, all of which are found in the Res subunit of the type III restriction enzymes. To determine the contribution, if any, of these motifs in DNA cleavage by