Auxinic Herbicide Resistance May Be Modulated at the Auxin-Binding Site in Wild Mustard (Sinapis arvensis L.): A Light Scattering Study

Author： deshpande S. Hall J.C.

Publisher： Academic Press

ISSN： 0048-3575

Source： Pesticide Biochemistry and Physiology, Vol.66, Iss.1, 2000-01, pp. : 41-48

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

The effects of IAA and the auxinic herbicides picloram and MCPP on the flash-induced nucleotide-independent light-scattering signal from picloram-resistant (R) and- susceptible (S) wild mustard (Sinapis arvensis L.) protoplasts were studied. We previously demonstrated that an increase in H+ efflux leads to a decrease in amplitude of the light-scattering signal. In this study, IAA increased H+ efflux (i.e., decreased the signal amplitude) in both R and S protoplasts in direct proportion to the IAA concentration. When the signal amplitudes from picloram-treated R and S biotype were compared, they were different. Picloram, which adversely affects the S biotype in vivo and in vitro, increased H+ efflux (reduced signal amplitude) in S protoplasts but did not affect H+ efflux in R. MCPP, which adversely affects both biotypes in vivo and in vitro, increased H+ efflux in both biotypes. Preincubation of S protoplasts with IAA did not alter the effects of picloram or MCPP on H+ efflux (i.e., increased efflux). Conversely, preincubation of R protoplasts with IAA reduced MCPP-mediated H+ efflux thereby reducing the adverse affects of MCPP. These results support our hypothesis that the physiological basis for this auxinic herbicide resistance in the wild mustard biotype is associated with their altered binding to auxin binding sites.

Related content

ATP-Dependent Auxin- and Auxinic Herbicide-Induced Volume Changes in Isolated Protoplast Suspensions from Sinapis arvensis L.

By deshpande S. Hall J.C.

Pesticide Biochemistry and Physiology, Vol. 56, Iss. 1, 1996-09 ,pp. : 26-43 (18)

Academic Press

Access to resources Recommend Favorite

Mechanism of Wild Oat (Avena fatua L.) Resistance to Imazamethabenz-Methyl

By Nandula V.K. Messersmith C.G.

Pesticide Biochemistry and Physiology, Vol. 68, Iss. 3, 2000-11 ,pp. : 148-155 (8)

Academic Press

Access to resources Recommend Favorite

Target-Site Resistance to Pyrethroids in Heliothis virescens (F.) and Helicoverpa zea (Boddie)

By Ottea J.A. Holloway J.W.

Pesticide Biochemistry and Physiology, Vol. 61, Iss. 3, 1998-09 ,pp. : 155-167 (13)

Academic Press

Access to resources Recommend Favorite

An isoleucine to leucine substitution in the ACCase of Alopecurus myosuroides (black-grass) is associated with resistance to the herbicide sethoxydim

By Brown A.C. Moss S.R. Wilson Z.A. Field L.M.

Pesticide Biochemistry and Physiology, Vol. 72, Iss. 3, 2002-03 ,pp. : 160-168 (9)

Academic Press

Access to resources Recommend Favorite

Herbicide Resistance and Supersensitivity in Ala₂₅₀ or Ala₂₅₁ Mutants of the D1 Protein in Chlamydomonas reinhardtii

By Johanningmeier U. Sopp G. Brauner M. Altenfeld U. Orawski G. Oettmeier W.

Pesticide Biochemistry and Physiology, Vol. 66, Iss. 1, 2000-01 ,pp. : 9-19 (11)

Academic Press

Access to resources Recommend Favorite