PRODUCTION OF IL-10, TNF-α, IFN-γ, TGF-β1 BY DIFFERENT POPULATIONS OF ERYTHROID CELLS DERIVED FROM HUMAN EMBRYONAL LIVER

Author： Sennikov S.V. Krysov S.V. Silkov A.N. Injelevskaya T.V. Kozlov V.A.

Publisher： Academic Press

ISSN： 1043-4666

Source： Cytokine, Vol.17, Iss.4, 2002-02, pp. : 221-225

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Abstract

It has previously been determined that erythroid cells of mice are capable of expressing such cytokines as interleukin (IL) 1α and β, IL-4, IL-6, interferon gamma (IFN-γ), granulocyte–macrophage colony-stimulating factor (GM-CSF) and transforming growth factor beta (TGF-β). It has been shown that glycophorin A⁺ (GlA⁺) and antigen erythroblasts (AG-EB⁺) (both human erythroid cells of embryonic origin) are also capable of producing a series of cytokines such as IL-1β, IL-2, IL-4 and IL-6. The aim of this work was to study the capacity of erythroid cells from human embryonic liver to produce such cytokines as IFN-γ, TGF-β1, tumour necrosis factor alpha (TNF-α) and IL-10. The erythroid cells were isolated by means of antibodies specific to erythroblasts (GlA and AG-EB), as well as those from single erythroid colonies. The production level of some cytokines varies insignificantly under the action of erythropoietin (Epo) and quantitatively differs in GlA⁺ and AG-EB⁺ erythroid cells. Hence, the erythroid cells express IFN-γ, TGF-β1, TNF-α and IL-10. The erythroid cells could be involved through the production of these cytokines in the regulation of such processes as self-renewal, proliferation and differentiation of cells of other blood-forming sites. .