Human cytokines interleukin (IL)-3 and IL-6 affect the growth and insulin binding of the unicellular organism Tetrahymena

Author: Csaba G.   Kovacs P.   Falus A.  

Publisher: Academic Press

ISSN: 1043-4666

Source: Cytokine, Vol.7, Iss.8, 1995-11, pp. : 771-774

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Abstract

Interleukin (IL)-3 and IL-6 significantly increase the growth rate of the unicellular organism, Tetrahymena . The effect elicited by IL-3 is long lasting as it was also detectable after 20 generations. Effect of IL-6 was detectable as long as the substance was present in the cell culture. Pretreatment with IL-3 did not enhance the proliferative response to subsequent IL-3 treatment, but the second exposure to IL-3 considerably depressed the active proliferation of Tetrahymena cells. However, a positive `priming effect' elicited by IL-6 resulted in an increased growth rate following repeated IL-6 stimulation. Insulin binding to the plasma membrane of Tetrahymena was increased by IL-6 but not by IL-3 after 24 hours, and this enhancement appeared even after one hour incubation. If the cells were pretreated with insulin, IL-6 did not influence insulin binding, while an inhibition by IL-3 was observed. These results direct attention to the similarities of actions induced by IL-3 and IL-6 at different levels of phylogeny probably due to the presence of cytokine receptor-like structures on this unicellular organism.

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