S-Layer Stabilized Solid Supported Lipid Bilayers

ISSN： 1047-8477

Source： Journal of Structural Biology, Vol.119, Iss.2, 1997-07, pp. : 123-128

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Abstract

This work describes composite structures composed of lipid bilayer or tetraetherlipid monolayer films attached to solid supports with associated crystalline bacterial cell surface layers (S-layers). The bilayer system was established by making use of the strong chemisorption of a first monolayer of thiolipids (1-octadecanethiol or 1,2-dimyristoyl sn -glycero-3-phosphothioethanol) on gold and attaching a second monolayer of 1,2-dipalmitoyl- sn -3-phosphatidylethanolamine by the Langmuir Schaefer technique. The tetraetherlipid monolayer was composed of Glycerol-dialkyl-nonitol tetraetherlipid (GDNT). The monolayer of GDNT exhibits the thickness of a bilayer with hydrophilic headgroups on both sides and a hydrophobic inner part. Isolated S-layer protein from Bacillus sphaericus CCM2177, which was injected into the subphase of an LB-trough, recrystallized into a coherent monolayer at the solid supported phospholipid bilayer and at the tetraetherlipid monolayer. The composite lipid/S-layer structures were stable enough to allow lifting from the air-water interface, rinsing in water, and transfer into a scanning force microscope.