Author： Hofmann U.G. Rotsch C. Parak W.J. Radmacher M.
Publisher： Academic Press
ISSN： 1047-8477
Source： Journal of Structural Biology, Vol.119, Iss.2, 1997-07, pp. : 84-91
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
We have investigated living chicken cardiocytes with an atomic force microscope (AFM). Cytoskeletal structures like stress fibers can easily be imaged with the AFM. Here we have also measured the cell's elastic properties. By taking force curves as a function of lateral position (force mapping) we could compare the elastic properties at different locations of the same cell. In the lamellipodal region investigated here in detail, the elastic moduli range from around 10 up to 200 kPa on top of stress fibers. By degradation with cytochalasin B we can estimate to what extent the elastic properties of this type of cell are determined by the actin network.
Related content
Access to resources
Recommend
By Muller D.J. Schoenenberger C.A. Schabert F. Engel A.
Journal of Structural Biology, Vol. 119, Iss. 2, 1997-07 ,pp. :
Hyaluronic Acid by Atomic Force Microscopy
By Jacoboni I. Valdrè U. Mori G. Quaglino D. Pasquali-Ronchetti I.
Journal of Structural Biology, Vol. 126, Iss. 1, 1999-06 ,pp. :