Low-Dose Automated Electron Tomography: A Recent Implementation

Author: Rath B.K.   Marko M.   Radermacher M.   Frank J.  

Publisher: Academic Press

ISSN: 1047-8477

Source: Journal of Structural Biology, Vol.120, Iss.3, 1997-12, pp. : 210-218

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Abstract

Low-dose automated tomography has been implemented on a 400-kV JEOL intermediate voltage electron microscope. Instrumentation and procedures for automatic tomographic series data collection are described. Difficulties encountered and ways to overcome them are discussed. A low-dose tomographic projection series of a triad junction of frog sartorius muscle was semiautomatically collected and a 3-D reconstruction of this organelle was made. Copyright 1997 Academic Press.

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