

Author: Bingle L.E.H. Simpson T.J. Lazarus C.M.
Publisher: Academic Press
ISSN: 1087-1845
Source: Fungal Genetics and Biology, Vol.26, Iss.3, 1999-04, pp. : 209-223
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Abstract
Analysis of fungal polyketide synthase gene sequences suggested that these might be divided into two subclasses, designated WA-type and MSAS-type. Two pairs of degenerate PCR primers (LC1 and LC2c, LC3 and LC5c) were designed for the amplification of ketosynthase domain fragments from fungal PKS genes in each of these subclasses. Both primer pairs were shown to amplify one or more PCR products from the genomes of a range of ascomycetous Deuteromycetes and Southern blot analysis confirmed that the products obtained with each pair of primers emanated from distinct genomic loci. PCR products obtained from
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