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Three-Dimensional Protein NMR TROSY-Type ¹⁵N-resolved ¹H^N–¹H^N NOESY Spectra with Diagonal Peak Suppression

Author： Meissner A. Sørensen O.W.

Publisher： Academic Press

ISSN： 1090-7807

Source： Journal of Magnetic Resonance, Vol.142, Iss.1, 2000-01, pp. : 195-198

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

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Abstract

An earlier two-dimensional NOESY experiment with diagonal peak suppression in the ¹H^N–¹H^N region is extended to three dimensions by including ¹⁵N evolution while maintaining the TROSY approach throughout. The technique suppresses all anti-TROSY resonances by appropriate pulse sequence elements and for large molecules at high fields possible semi- and anti-TROSY artifacts are further suppressed by virtue of much shorter transverse relaxation times for these components. The new technique is demonstrated using an ¹⁵N-labeled protein sample, RAP 17-97 (N-terminal domain of α2-macroglobulin Receptor Associated Protein), in H₂O at 500 MHz.

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