Author： Berensmeier Sonja Jördening Hans-Joachim Buchholz Klaus
Publisher： Informa Healthcare
ISSN： 1024-2422
Source： Biocatalysis and Biotransformation, Vol.24, Iss.4, 2006-07, pp. : 280-290
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Abstract
The acceptor reaction of dextransucrase from Leuconostoc mesenteroides NRRL-B512F with glucose as acceptor is of technical interest for isomaltooligosaccharide (IMOs) synthesis. Different experimental conditions were investigated for free and immobilized enzyme. The data for oligosaccharide formation up to a degree of polymerization 4 were correlated with a model developed earlier, and optimal reaction conditions for immobilized dextransucrase design and application were identified for later continuous application. Furthermore, stability was investigated for free and immobilized enzyme including stabilization by sugars.
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