Generation of hydrogen peroxide by “Antioxidant” beverages and the effect of milk addition. Is cocoa the best beverage?

Author: Long Lee   Lan Angeline   Hsuan Felicia   Halliwell Barry  

Publisher: Informa Healthcare

ISSN: 1071-5762

Source: Free Radical Research, Vol.31, Iss.1, 1999-01, pp. : 67-71

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Abstract

The ability of several beverages to generate hydrogen peroxide was demonstrated by direct measurement using the ferrous ion oxidation-xylenol orange (FOX) assay. Tea and coffee could generate H 2 O 2 to achieve levels over 100 μM, but cocoa did not. Milk decreased net H 2 O 2 production by beverages and showed some ability to remove H 2 O 2 itself, apparently not because of catalase activity. Hence several of the beverages commonly drunk by humans show a complex mixture of anti- and pro-oxidant abilities.