Nuclear DNA Synthesis in Yeast and the Effect of Irradiation

Author: Resnick Michael A.   Martin Patricia  

Publisher: Informa Healthcare

ISSN: 1362-3095

Source: International Journal of Radiation Biology, Vol.31, Iss.4, 1977-04, pp. : 365-375

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Abstract

The synthesis of DNA can be measured in yeast by following the uptake of 5-bromodeoxy-uridine-5′-triphosphate in a mutant that utilizes deoxythymidine-5′-monophosphate; approximately 60 per cent of the DNA is synthesized semi-conservatively before replication stops. Neither ultraviolet light (U.V.), nor ionizing radiation stimulates repair-type synthesis. Based on the ability to detect small amounts of synthesis, it appears that fewer than ten bases are synthesized per pyrimidine dimer removed.