Correlation between the Radiobiological Oxygen Constant, K, and the Non-protein Sulphydryl Content of Mammalian Cells

Author： Cullen Beulah M. Michalowski A. Walker Hilary C. Révész L.

ISSN： 1362-3095

Source： International Journal of Radiation Biology, Vol.38, Iss.5, 1980-11, pp. : 525-535

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Abstract

The value of the radiobiological oxygen constant K has been found to depend on the concentration of non-protein sulphydryl (NPSH) within the cell. Cells in the exponential phase of growth have a higher concentration of NPSH and a higher value of K than cells in plateau phase. Binding NPSH with N-ethylmaleimide reduced the value of K and conversely, addition of NPSH as dithioerythritol increased the value of K. K also rises with the same time course as NPSH increases, when plateau phase cells are replated into fresh medium. These results support the hypothesis that free -SH groups within the cell compete with oxygen to react with radiation damaged molecules.

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