DNA Double-strand Break Induction in Yeast by X-rays and α-particles Measured by Pulsed-field Gel Electrophoresis

Author： Löbrich M.   Ikpeme S.   Haub P.   Weber K.-J.   Kiefer J.  

Publisher： Informa Healthcare

ISSN： 1362-3095

Source： International Journal of Radiation Biology, Vol.64, Iss.5, 1993-11, pp. : 539-546

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

Pulsed-field gel electrophoresis was used to separate the chromosomes of the diploid yeast Saccharomyces cerevisiae 211 *B after irradiation with X-rays and α-particles. After electrophoresis, gels were stained with ethidium bromide, placed on a UV-transilluminator and photographed with a digitizing camera connected to a personal computer. The pictures obtained were processed with the help of specially developed software which allows for the correction of the camera's shading effect and background fluorescence. Linearity between DNA amount and fluorescence was demonstrated. Fluorescence intensity for the band with the lowest electrophoretic mobility was found to decrease exponentially with dose. Based on the known size of the native DNA molecules, double-strand break yields could be calculated. These were found to be (8·2 ± 0·4) and (14·8 ± 0·5)10−12 (g/mol)−1 Gy−1 for 80 keV X-rays and 3·5 MeV 241Am α-particles respectively which gives a relative biological effectiveness of 1·8 ± 0·1.

Related content

DNA Double-strand Break Induction and Rejoining as Determinants of Human Tumour Cell Radiosensitivity. A Pulsed-field Gel Electrophoresis Study

By Whitaker S.J.   Ung Y.C.   McMillan T.J.  

International Journal of Radiation Biology, Vol. 67, Iss. 1, 1995-01 ,pp. : 7-18 (12)

Informa Healthcare

Access to resources Recommend Favorite

Oxygen Effect for DNA Double-strand Break Induction Determined by Pulsed-field Gel Electrophoresis

By Whitaker S.J.   McMillan T.J.  

International Journal of Radiation Biology, Vol. 61, Iss. 1, 1992-01 ,pp. : 29-41 (13)

Informa Healthcare

Access to resources Recommend Favorite

A Comparison of Methods for Calculating DNA Double-strand Break Induction Frequency in Mammalian Cells by Pulsed-field Gel Electrophoresis

By de Almodóvar J.M. Ruiz   Steel G.G.   Whitaker S.J.   McMillan T.J.  

International Journal of Radiation Biology, Vol. 65, Iss. 6, 1994-06 ,pp. : 641-649 (9)

Informa Healthcare

Access to resources Recommend Favorite

DNA Double-strand Break Measurement in Mammalian Cells by Pulsed-field Gel Electrophoresis: An Approach Using Restriction Enzymes and Gene Probing

By Löbrich M.   Ikpeme S.   Kiefer J.  

International Journal of Radiation Biology, Vol. 65, Iss. 6, 1994-06 ,pp. : 623-630 (8)

Informa Healthcare

Access to resources Recommend Favorite

DNA Double-strand Breaks Measured by Pulsed-field Gel Electrophoresis in Irradiated Lymphocytes from Normal Humans and Those with Alzheimer's Disease

By Tobi S.E.   Itzhaki R.F.  

International Journal of Radiation Biology, Vol. 63, Iss. 5, 1993-05 ,pp. : 617-622 (6)

Informa Healthcare

Access to resources Recommend Favorite