Author： Xiao Y. Natarajan A. T.
Publisher： Informa Healthcare
ISSN： 1362-3095
Source： International Journal of Radiation Biology, Vol.75, Iss.8, 1999-08, pp. : 943-951
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Abstract
Purpose: To study by fluorescence in situ hybridization (FISH) the involvement of Chinese hamster chromosomes 3, 4, 8, and 9, and their separate chromosome arms in X-ray-induced aberrations. Materials and methods: Male embryonic primary cells of Chinese hamster were used and metaphases were collected and scored at 20h after confluent cells were exposed to 1 and 4Gy X-rays. The frequencies and types of chromosomal aberrations involving chromosomes 3, 4, 8, and 9 were studied by FISH using armspecific painting probes. Proportional distribution of the induced aberrations was tested on the basis of the relative lengths of chromosomes or chromosome arms studied. Results: A non-proportional distribution of breaks, colour junctions as well as apparently simple dicentrics and translocations among the chromosomes studied was observed in the 4Gy group. In general, chromosome 3 was less involved than expected and chromosome 8 was more involved than expected, and chromosomes 4 and 9 were involved as expected. A non-proportional involvement of arms in breaks, colour junctions and apparently simple dicentrics was also observed. The short arm of chromosome 3 was more involved in breaks and colour junctions than expected. The long arm of chromosome 4 was more involved in dicentrics than expected. Conclusions: Results of this study indicate a non-proportional involvement of Chinese hamster chromosomes 3, 4, 8 and 9 as well as their arms in different types of aberrations following irradiation.
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