High prevalence of chromosome 10 rearrangements in human lymphocytes after in vitro X-ray irradiation

Author： Scarpato R.   Lori A.   Tomei A.   Cipollini M.   Barale R.  

Publisher： Informa Healthcare

ISSN： 1362-3095

Source： International Journal of Radiation Biology, Vol.76, Iss.5, 2000-05, pp. : 661-666

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Abstract

Purpose: To evaluate the chromosome symmetric or asymmetric rearrangement (CR) frequency for chromosome 10 compared to chromosomes 1 and 3 induced in vitro in human lymphocytes by low doses of X-rays. Materials and methods: Blood samples obtained from three young and healthy volounteers were irradiated in G0 with 0.25, 0.50 and 1 Gy X-rays. Chromosome painting analysis was used on preparations of peripheral lymphocytes for the identification of CR.Results: It was found that radiation-induced CR levels were non-randomly distributed among the three painted chromosomes. Chromosome 10 CR frequencies were significantly greater than those involving chromosomes 1 (at all the doses tested) or 3 (at 0.25 and 1 Gy), with frequency ratios ranging from 2.2 to 5.2. Conclusions: In comparison to chromosomes 1 and 3, chromosome 10 appeared to be involved in exchanging at a significantly greater extent than expected according to its DNA content.

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