Association of HLA-DRB1**14 with rheumatic heart disease patients from Chandigarh, North India

Author： Toor Devinder Leal Komal Kumar Rajesh Sharma Yash Paul Chakraborti Anuradha

ISSN： 1366-5804

Source： Biomarkers, Vol.17, Iss.2, 2012-03, pp. : 160-165

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Abstract

Context: Acute rheumatic fever (ARF)/rheumatic heart disease (RHD) is a major cause of morbidity and mortality in India, yet, few studies are available on susceptibility markers.Objective: To associate human leukocyte antigen (HLA) class II alleles with north Indian RHD patients as genetic susceptibility markers.Materials and methods: HLA alleles were analysed using sequence specific primer-polymerase chain reaction and nucleotide sequencing, while HLA-B27 expression by flowcytometry.Results: Few HLA-DQB1/DRB1 alleles were associated with RHD and HLA-DRB1**14 gene polymorphism revealed two single nucleotide polymorphisms (SNPs) in patients. Bioinformatic predictions showed influence of SNPs on protein function. HLA-B27 was positive in 42.85% ARF patients.Conclusion: The study showed association of different HLA class II alleles with RHD in North Indian population.