Methylglyoxal causes structural and functional alterations in adipose tissue independently of obesity

Author： Matafome P. Santos-Silva D. Crisóstomo J. Rodrigues T. Rodrigues L. Sena C. M. Pereira P. Seiça R.

ISSN： 1381-3455

Source： Archives of Physiology and Biochemistry, Vol.118, Iss.2, 2012-05, pp. : 58-68

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

Context:Adipose tissue is one of the first organs to develop insulin resistance even with moderate BMI. However, the contribution of developing hyperglycaemia and concomitant methylglyoxal increment to tissue dysfunction during type 2 diabetes progression was not addressed before.Methods:Young and aged Wistar and Goto-Kakizaki rats (non-obese model of type 2 diabetes) and a group of MG-treated W rats were used to investigate the chronic effects of hyperglycaemia and ageing and specifically MG-induced mechanisms.Results:Diabetic and aged rats showed decreased adipose tissue irrigation and interstitial hypoxia. Hyperglycaemia of diabetic rats leaded to fibrosis and accumulation of PAS-positive components, exacerbated in aged animals, which also showed decreased hipoadiponectinemia, increased MCP-1 expression and macrophage infiltration to glycated fibrotic regions. MG leaded to increased free fatty acids, hipoadiponectinemia, decreased irrigation, hypoxia and macrophage recruitment for glycated fibrotic regions.Conclusions:MG contributes to dysfunction of adipose tissue during type 2 diabetes progression.