Description
High-Performance Liquid Chromatography of Proteins and Peptides contains the proceedings of the first International Symposium on High-Performance Liquid Chromatography of Proteins and Peptides, held in Washington, D.C., on November 16-17, 1981. The symposium focused on the use of high-performance liquid chromatography (HPLC) in the analysis, characterization, and isolation of peptides and proteins and encompassed six sessions covering size exclusion, ion exchange, and reversed phase chromatography, as well as the use of high-performance liquid chromatography (HPLC) in protein structural studies and peptide isolation.
This book is comprised of 28 chapters and begins with a discussion on the status of high-performance ion-exchange chromatography of proteins, followed by an analysis of peptic fragmentation of human immunoglobulin G using HPLC. The physicochemical basis of peptide retention with chemically bonded hydrocarbonaceous silicas and the isolation of biologically active peptides from tissue extracts are also examined. Subsequent chapters explore some additional applications of HPLC, such as cord blood screening for hemoglobin disorders; purification of commercial trypsin and chymotrypsin; characterization of human alcohol dehydrogenase isoenzymes; and structural studies of neurophysins, photolabeled derivatives, and biosynthetic precursors.
This monograph should be of value to students and researchers interested in the use of HPLC to study proteins and pep
Chapter
Chapter 1. High-Performance Ion-Exchange Chromatography of Proteins: The Current Status1
pp.：
18 – 26
Chapter 2. Analysis of Peptic Fragmentation of Human Immunoglobulin G Using High-Performance Liquid Chromatography*
pp.：
26 – 34
Chapter 3. Cord Blood Screening for Hemoglobin Disorders by High-Performance Liquid Chromatography*·1
pp.：
34 – 40
Chapter 4. A Simple and Rapid Purification of Commercial Trypsin and Chymotrypsin by Reverse-Phase High-Performance Liquid Chromatography*
pp.：
40 – 46
Chapter 5. Adaptation of Reverse-Phase High-Performance Liquid Chromatography for the Isolation and Sequence Analysis of Peptides from Plasma Amyloid P-Component*·1
pp.：
46 – 56
Chapter 6. Characterization of Human Alcohol Dehydrogenase Isoenzymes by High- Performance Liquid Chromatographie Peptide Mapping*
pp.：
56 – 64
Chapter 7. Purification of Glycopeptides of Human Ceruloplasmin and Immunoglobulin D by High-Pressure Liquid Chromatography*
pp.：
64 – 72
Chapter 8. High-Performance Size-Exclusion Chromatography of Hydrolyzed Plant Proteins*
pp.：
72 – 82
Chapter 9. The Isolation of Peptides by High-Performance Liquid Chromatography Using Predicted Elution Positions*
pp.：
82 – 90
Chapter 10. The Application of High-Performance Liquid Chromatography for the Resolution of Proteins Encoded by the Human Adenovirus Type 2 Cell Transformation Region1
pp.：
90 – 98
Chapter 11. The Importance of Silica Type for Reverse-Phase Protein Separations*·1
pp.：
98 – 112
Chapter 12. Variables in the High-Pressure Cation-Exchange Chromatography of Proteins*
pp.：
112 – 120
Chapter 13. Application of High-Performance Liquid Chromatography to Competitive Labeling Studies: The Chemical Properties of Functional Groups of Glucagon*
pp.：
120 – 128
Chapter 14. Purification by Reverse-Phase High-Performance Liquid Chromatography of an Epidermal Growth Factor-Dependent Transforming Growth Factor*
pp.：
128 – 136
Chapter 15. Preparative Size-Exclusion Chromatography of Human Serum Apolipoproteins Using an Analytical Liquid Chromatograph1
pp.：
136 – 146
Chapter 16. Use of Reverse-Phase High-Performance Liquid Chromatography in Structural Studies of Neurophysins, Photolabeled Derivatives, and Biosynthetic Precursors1
pp.：
146 – 158
Chapter 17. Purification of Radiolabeled and Native Polypeptides by Gel Permeation High-Performance Liquid Chromatography1
pp.：
158 – 168
Chapter 18. Nonideal Size-Exclusion Chromatography of Proteins: Effects of pH at Low Ionic Strength1,2
pp.：
168 – 178
Chapter 19. Factors Influencing Chromatography of Proteins on Short Alkylsilane-Bonded Large Pore-Size Silicas1
pp.：
178 – 190
Chapter 20. High-Performance Liquid Chromatography Analysis in the Synthesis, Characterization, and Reactions of Neoglycopeptides
pp.：
190 – 198
Chapter 21. Use of Size-Exclusion High-Performance Liquid Chromatography for the Analysis of the Activation of Prothrombin1,2
pp.：
198 – 206
Chapter 22. High-Performance Liquid Chromatography of Proteins: Purification of the Acidic Isozyme of Adenylosuccinate Synthetase from Rat Liver1
pp.：
206 – 212
Chapter 23. Application of High-Performance Liquid Chromatographie Peptide Purification to Protein Microsequencing by Solid-Phase Edman Degradation1
pp.：
212 – 228
Chapter 24. Measurement of Endogenous Leucine Enkephalin in Canine Caudate Nuclei and Hypothalami with High-Performance Liquid Chromatography and Field-Desorption Mass Spectrometry1
pp.：
228 – 238
Chapter 25. Fluorescent Techniques for the Selective Detection of Chromatographically Separated Peptides1
pp.：
238 – 250
Chapter 26. High-Performance Liquid Chromatographie Purification of Peptide Hormones: Ovine Hypothalamic Amunine (Corticotropin Releasing Factor)1
pp.：
250 – 260
Chapter 27. Isolation and Purification of Escherichia coli Heat-Stable Enterotoxin of Porcine Origin1
pp.：
260 – 270
Chapter 28. α -N-Acetyl- β -Endorphin1_26 from the Neurointermediary Lobe of the Rat Pituitary: Isolation, Purification, and Characterization by High-Performance Liquid Chromatography1
pp.：
270 – 280
High-Performance Liquid Chromatography of Proteins and Peptides
：Proceedings of the first International Symposium
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