Electron Microscopy of Plant Cells

Author: Hawes   C  

Publisher: Elsevier Science‎

Publication year: 2012

E-ISBN: 9780323148986

P-ISBN(Paperback): 9780123188809

P-ISBN(Hardback):  9780123188809

Subject: Q94 Botany

Language: ENG

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Description

Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens.
Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others.
This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.

Chapter

Front Cover

pp.:  1 – 4

Copyright Page

pp.:  5 – 8

Contributors

pp.:  6 – 10

Table of Contents

pp.:  8 – 6

Preface

pp.:  10 – 12

Chapter 2. Stereo-electron microscopy

pp.:  78 – 96

Chapter 3. Quantitative Morphological Analysis

pp.:  96 – 116

Chapter 4. Enzyme Cytochemistry

pp.:  116 – 192

Chapter 5. Immunogold Labelling

pp.:  192 – 230

Chapter 6. In situ Hybridization Techniques: Molecular Cytology Goes Ultrastructural

pp.:  230 – 268

Chapter 7. Rapid-Freezing Methods and their Applications

pp.:  268 – 324

Chapter 8. Ambient- and Low-Temperature Scanning Electron Microscopy

pp.:  324 – 426

Chapter 9. Microanalysis

pp.:  426 – 468

Index

pp.:  468 – 478

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