Cryo-EM Part B: 3-D Reconstruction ：3-D Reconstruction ( Volume 482 )

Publication subTitle ：3-D Reconstruction

Publication series ：Volume 482

Author： Jensen   Grant  

Publisher： Elsevier Science‎

Publication year： 2010

E-ISBN: 9780123849922

P-ISBN(Paperback): 9780123849915

P-ISBN(Hardback):  9780123849915

Subject： Q5 Biochemistry;Q7 Molecular Biology

Language： ENG

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Description

This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

* Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide

Chapter

Front Cover

pp.：  1 – 4

Methods in Enzymology Cryo-EM, Part B: 3-D Reconstruction

pp.：  4 – 5

Copyright page

pp.：  5 – 6

Contents

pp.：  6 – 12

Contributors

pp.：  12 – 16

Preface

pp.：  16 – 18

Methods in Enzymology

pp.：  18 – 48

Chapter 1: Fundamentals of Three-Dimensional Reconstruction from Projections

pp.：  48 – 82

Chapter 2: Image Restoration in Cryo-Electron Microscopy

pp.：  82 – 120

Chapter 3: Resolution Measures in Molecular Electron Microscopy

pp.：  120 – 148

Chapter 4: 3D Reconstruction from 2D Crystal Image and Diffraction Data

pp.：  148 – 178

Chapter 5: Fourier-Bessel Reconstruction of Helical Assemblies

pp.：  178 – 214

Chapter 6: Reconstruction of Helical Filaments and Tubes

pp.：  214 – 232

Chapter 7: Three-Dimensional Asymmetric Reconstruction of Tailed Bacteriophage

pp.：  232 – 258

Chapter 8: Single Particle Analysis at High Resolution

pp.：  258 – 284

Chapter 9: The Orthogonal Tilt Reconstruction Method

pp.：  284 – 310

Chapter 10: An Introduction to Maximum-Likelihood Methods in Cryo-EM

pp.：  310 – 342

Chapter 11: Classification of Structural Heterogeneity by Maximum-Likelihood Methods

pp.：  342 – 368

Chapter 12: Methods for Three-Dimensional Reconstruction of Heterogeneous Assemblies

pp.：  368 – 390

Chapter 13: Alignment of Cryo-Electron Tomography Datasets

pp.：  390 – 416

Chapter 14: Correcting for the Ewald Sphere in High-Resolution Single-Particle Reconstructions

pp.：  416 – 428

Chapter 15: Software Tools for Molecular Microscopy: An Open-Text Wikibook

pp.：  428 – 440

Author Index

pp.：  440 – 450

Subject Index

pp.：  450 – 458

Colour Plate

pp.：  458 – 473

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