Thiol Redox Transitions in Cell Signaling, Part A :Chemistry and Biochemistry of Low Molecular Weight and Protein Thiols ( Volume 473 )

Publication subTitle :Chemistry and Biochemistry of Low Molecular Weight and Protein Thiols

Publication series :Volume 473

Author: Cadenas   Enrique;Packer   Lester  

Publisher: Elsevier Science‎

Publication year: 2010

E-ISBN: 9780123813466

P-ISBN(Paperback): 9780123813459

P-ISBN(Hardback):  9780123813459

Subject: Q2 Cytobiology;Q5 Biochemistry

Language: ENG

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Description

Thiol Redox Transitions in Cell Signaling, Part A, along with its companion (volume 475), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. This first installment of Cadenas and Packer's two-volume treatment specifically deals with glutathionylation and dethiolation, and peroxide removal by peroxiredoxins/thioredoxins and glutathione peroxidases.

The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Over 450 volumes have been published to date, and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

  • Along with companion volume, provides a full overview of techniques necessary to the study of thiol redox in relation to cell signaling
  • Gathers tried and tested techniques from global labs, offering both new and tried-and-true methods
  • Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Chapter

Front Cover

pp.:  1 – 4

Copyright Page

pp.:  5 – 6

Contents

pp.:  6 – 12

Contributors

pp.:  12 – 20

Preface

pp.:  20 – 22

Volumes in Series

pp.:  22 – 50

Chapter 1: Changing Paradigms in Thiology: From Antioxidant Defense Toward Redox Regulation

pp.:  50 – 90

Chapter 2: Mass Spectrometry-Based Methods for the Determination of Sulfur and Related Metabolite Concentrations in Cell Extracts

pp.:  90 – 126

Chapter 3: Use of Dimedone-Based Chemical Probes for Sulfenic Acid Detection: Evaluation of Conditions Affecting Probe Incorporation into Redox-Sensitive Proteins

pp.:  126 – 144

Chapter 4: Use of Dimedone-Based Chemical Probes for Sulfenic Acid Detection: Methods to Visualize and Identify Labeled Proteins

pp.:  144 – 166

Chapter 5: Formation and Reactions of Sulfenic Acid in Human Serum Albumin

pp.:  166 – 186

Chapter 6: Determination of GSH, GSSG, and GSNO Using HPLC with Electrochemical Detection

pp.:  186 – 198

Chapter 7: Measurement of Mixed Disulfides Including Glutathionylated Proteins

pp.:  198 – 210

Chapter 8: Detection and Quantification of Protein Disulfides in Biological Tissues: A Fluorescence-BasedProteomic Approach

pp.:  210 – 228

Chapter 9: Measurement and Identification of S-Glutathiolated Proteins

pp.:  228 – 248

Chapter 10: Proteome Screens for Cys Residues Oxidation: The Redoxome

pp.:  248 – 266

Chapter 11: Identification by MS/MS of Disulfides Produced by a Functional Redox Transition

pp.:  266 – 276

Chapter 12: Mass Spectrometry Approaches for the Redox Characterization of Protein Cysteine Residues: The Case of theTranscription Factor Pax-8

pp.:  276 – 300

Chapter 13: A Simple Method to Systematically Study Oxidatively Modified Proteins in Biological Samples and Its Applications

pp.:  300 – 314

Chapter 14: Direct and Indirect Detection Methods for the Analysis of S-Nitrosylated Peptides and Proteins

pp.:  314 – 330

Chapter 15: A Rapid Approach for the Detection, Quantification, and Discovery of Novel Sulfenic Acid or S-Nitrosothiol Modified Proteins Using a Biotin-Switch Method

pp.:  330 – 354

Chapter 16: Protein Adducts of Aldehydic Lipid Peroxidation Products: Identification and Characterizationof Protein Adducts Using anAldehyde/Keto-Reactive Probein Combination with MassSpectrometry

pp.:  354 – 380

Author Index

pp.:  380 – 402

Subject Index

pp.:  402 – 410

Color Plate

pp.:  410 – 412

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