Flow Cytogenetics ( Analytical Cytology )

Publication series ：Analytical Cytology

Author： Ploem   Johan S.;Gray   Joe W.  

Publisher： Elsevier Science‎

Publication year： 2012

E-ISBN: 9780080984520

P-ISBN(Paperback): 9780122961106

P-ISBN(Hardback):  9780122961106

Subject： Q3 Genetics;Q7 Molecular Biology

Language： ENG

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Description

This is the first book to be devoted entirely to the application and development of flow techniques in cytogenetics. It provides comprehensive information on the use of flow cytometry and sorting for chromosome classification and purification. Cytogenetics and molecular biologists will find this book an invaluable reference source.

  • Practical details for the preparation and analysis of chromosomes using flow cytometry
  • Flow karyotyping for sensitive rapid analysis of chromosome normality and the detection of aberrant chromosomes
  • Flow sorting as a source of chromosome-specific DNA for gene mapping and recombinant DNA libraries
  • Construction and current status of chromosome-specific recombinant DNA libraries

Chapter

Front Cover

pp.：  1 – 4

Flow Cytogenetics

pp.：  4 – 5

Copyright Page

pp.：  5 – 6

Table of Contents

pp.：  6 – 12

Contributors

pp.：  12 – 16

Preface

pp.：  16 – 18

Chapter 1. Human Chromosome Analysis by Flow Cytometry

pp.：  18 – 34

Chapter 2. Instrumentation for Chromosome Analysis and Sorting

pp.：  34 – 52

Chapter 3. Cell Culture for Chromosome Isolation

pp.：  52 – 60

Chapter 4. Chromosome Isolation Procedures

pp.：  60 – 78

Chapter 5. DNA Stains as Cytochemical Probes for Chromosomes

pp.：  78 – 100

Chapter 6. Methods for Estimating Components of Multipeaked Flow Histograms

pp.：  100 – 130

Chapter 7. Univariate Flow Karyotype Analysis

pp.：  130 – 154

Chapter 8. Bivariate Flow Karyotyping

pp.：  154 – 168

Chapter 9. Relation Between Radiation-induced Flow Karyotype Changes Analysed by Fourier Analysis and Chromosome Aberrations

pp.：  168 – 178

Chapter 10. Detection of Randomly Occurring Aberrant Chromosomes as a Measure of Genetic Change

pp.：  178 – 190

Chapter 11. Chromosome Classification by Scanning Flow Cytometry

pp.：  190 – 208

Chapter 12. Prepurification of Chromosomes by Velocity Sedimentation and Applications

pp.：  208 – 228

Chappter 13. Chromosome Purification by High-speed Sorting

pp.：  228 – 242

Chapter 14. Gene Mapping Strategies and Bivariate Flow Cytogenetics

pp.：  242 – 260

Chapter 15. Metaphase Chromosome Flow Sorting and Cloning; Rationale, Approaches and Applications

pp.：  260 – 274

Chapter 16. The National Laboratory Gene Library Project

pp.：  274 – 292

Chapter 17. Flow Cytometric Measurement of Specific DNA and RNA Sequences

pp.：  292 – 322

Index

pp.：  322 – 332

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