Calcium in Living Cells ( 2 )

Publication series :2

Author: Whitaker   Michael  

Publisher: Elsevier Science‎

Publication year: 2010

E-ISBN: 9780080951225

P-ISBN(Paperback): 9780123748416

P-ISBN(Hardback):  9780123748416

Subject: Q26 cellular biochemistry

Language: ENG

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Description

Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases.

  • Gives researchers much needed information on how to study calcium in live cells, which is becoming increasingly important in heart, musculoskeletal, and immune system research
  • Provides an overview of the latest methods--fluorescence resonance energy transfer (FRET), for example-- that are new to the field
  • Allows understanding of how calcium plays a role in intracellular function at the cellular level, which has proved important in Alzheimer's research, heart disease, and areas of musculoskeletal research
  • Updated chapters reflect advancements in the classic techniques used'preparing calcium buffers, vibrating the Ca2+ Electrode and confocal imaging

Chapter

Front Cover

pp.:  1 – 4

Methods in Cell Biology

pp.:  4 – 5

Copyright Page

pp.:  5 – 6

Contents

pp.:  6 – 10

Contributors

pp.:  10 – 12

Preface

pp.:  12 – 14

Chapter 2: Photorelease Techniques for Raising or Lowering Intracellular Ca2+

pp.:  40 – 80

Chapter 3: Making and Using Calcium-Selective Mini- and Microelectrodes

pp.:  80 – 104

Chapter 4: Construction, Theory, and Practical Considerations for using Self-referencing of Ca2+-Selective Microelectrodes forMonitoring Extracellular Ca2+ Gradients

pp.:  104 – 126

Chapter 5: Practical Aspects of Measuring Intracellular Calcium Signals with Fluorescent Indicators

pp.:  126 – 166

Chapter 6: Genetically Encoded Probes for Measurement of Intracellular Calcium

pp.:  166 – 196

Chapter 7: Patch Clamp Methods for Studying Calcium Channels

pp.:  196 – 212

Chapter 8: Nuclear Patch-Clamp Recording from Inositol 1,4,5-Trisphosphate Receptors

pp.:  212 – 238

Chapter 9: Confocal and Multiphoton Imaging of Intracellular Ca2+

pp.:  238 – 276

Chapter 10: The Use of Aequorins to Record and Visualize Ca2+ Dynamics: From Subcellular Microdomains to Whole Organisms

pp.:  276 – 314

Index

pp.:  314 – 320

Volumes in Series

pp.:  320 – 330

Color Plates

pp.:  330 – 338

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