Automated DNA Sequencing and Analysis

Author: Adams   Mark D.;Fields   Chris;Venter   J. Craig  

Publisher: Elsevier Science‎

Publication year: 2012

E-ISBN: 9780080926391

P-ISBN(Paperback): 9780127170107

P-ISBN(Hardback):  9780127170107

Subject: Q523 deoxyribonucleic acid (DNA)

Language: ENG

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Description

A timely book for DNA researchers, Automated DNA Sequencing and Analysis reviews and assesses the state of the art of automated DNA sequence analysis-from the construction of clone libraries to the developmentof laboratory and community databases. It presents the methodologies and strategies of automated DNA sequence analysis in a way that allows them to be compared and contrasted. By taking a broad view of the process of automated sequence analysis, the present volume bridges the gap between the protocols supplied with instrument and reaction kits and the finalized data presented in the research literature. It will be an invaluable aid to both small laboratories that are interested in taking maximum advantageof automated sequence resources and to groups pursuing large-scale cDNA and genomic sequencing projects.

  • The field of automation in DAN sequencing and analysis is rapidly moving, this book fulfils those needs, reviews the history of the art and provides pointers to future development.

Chapter

Front Cover

pp.:  1 – 4

Copyright Page

pp.:  5 – 10

Preface

pp.:  6 – 8

Acknowledgements

pp.:  8 – 22

Table of Contents

pp.:  10 – 6

PART I: SEQUENCING INSTRUMENTS AND STRATEGIES

pp.:  22 – 102

A. LIBRARIES

pp.:  102 – 152

PART II: Sample Preparation and Sequencing Methods

pp.:  102 – 102

B. AUTOMATED SAMPLE PREPARATION

pp.:  152 – 178

C. SEQUENCING METHODS

pp.:  178 – 238

A. SEQUENCE ASSEMBLY THEORY AND ALGORITHMS

pp.:  238 – 282

PART III: INFORMATICS

pp.:  238 – 238

B. DATA ANALYSIS TOOLS

pp.:  282 – 348

C. DATA MANAGEMENT AND DATABASES

pp.:  348 – 390

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