Description
The use of thermodynamics in biological research can be equated to an energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look to answer: What are the sources of energy that drive the function? Which of the pathways are of biological significance? As the base of macromolecular structures continues to expand through powerful techniques of molecular biology, such as X-ray crystal data and spectroscopy methods, the importance of tested and reliable methods for answering these questions will continue to expand as well. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions.
* Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules * Provides a "must-have" methods volume that keeps MIE buyers and online subscribers up-to-date with the latest research * Offers step-by-step lab instructions, including necessary equipment, from a global research community
Chapter
Chapter 1: Using NMR-Detected Backbone Amide 1H Exchange to assess Macromolecular Crowding Effects on Globular-Protein Stability
pp.：
50 – 68
Chapter 2: Fluorescence Spectroscopy in Thermodynamic and Kinetic Analysis of pH-Dependent Membrane Protein Insertion
pp.：
68 – 92
Chapter 3: Evaluating the Energy-Dependent ``Binding´´ in the Early Stage of Protein Import into Chloroplasts
pp.：
92 – 114
Chapter 4: Use of DNA Length Variation to Detect Periodicities in Positively Cooperative, Nonspecific Binding
pp.：
114 – 132
Chapter 5: The Impact of Ions on Allosteric Functions in Human Liver Pyruvate Kinase
pp.：
132 – 158
Chapter 6: Conformational Stability of Cytochrome c Probed by Optical Spectroscopy
pp.：
158 – 204
Chapter 7: Examining Ion Channel Properties Using Free-Energy Methods
pp.：
204 – 228
Chapter 8: Examining Cooperative Gating Phenomena in Voltage-Dependent Potassium Channels: Taking the Energetic Approach
pp.：
228 – 260
Chapter 9: Thermal Stability of Collagen Triple Helix
pp.：
260 – 282
Chapter 10: Electrostatic Contributions to the Stabilities of Native Proteins and Amyloid Complexes
pp.：
282 – 308
Chapter 11: Kinetics of Allosteric Activation
pp.：
308 – 322
Chapter 12: Thermodynamics of the Protein Translocation
pp.：
322 – 342
Chapter 13: Thermodynamic Analysis of the Structure-Function Relationship in the Total DNA-Binding Site of Enzyme-DNA Complexes
pp.：
342 – 374
Chapter 14: Equilibrium and Kinetic Approaches for Studying Oligomeric Protein Folding
pp.：
374 – 408
Chapter 15: Methods for Quantifying T cell Receptor Binding Affinities and Thermodynamics
pp.：
408 – 432
Chapter 16: Thermodynamic and Kinetic Analysis of Bromodomain-Histone Interactions
pp.：
432 – 458
Chapter 17: Thermodynamics of 2-Cys Peroxiredoxin Assembly Determined by Isothermal Titration Calorimetry
pp.：
458 – 480
Chapter 18: Protein-Lipid Interactions: Role of Membrane Plasticity and Lipid Specificity on Peripheral Protein Interactions
pp.：
480 – 504
Chapter 19: Predicting pKa Values with Continuous Constant pH Molecular Dynamics
pp.：
504 – 526
Chapter 20: Unfolding Thermodynamics of DNA Intramolecular Complexes Involving Joined Triple- and Double-Helical Motifs
pp.：
526 – 552
Chapter 21: Thermodynamics and Conformational Change Governing Domain-Domain Interactions of Calmodulin
pp.：
552 – 576
Chapter 22: Use of Pressure Perturbation Calorimetry to Characterize the Volumetric Properties of Proteins
pp.：
576 – 598
Chapter 23: Solvent Denaturation of Proteins and Interpretations of the m Value
pp.：
598 – 616
Chapter 24: Measuring Cotranslational Folding of Nascent Polypeptide Chains on Ribosomes
pp.：
616 – 640
Author Index
pp.：
640 – 652
Subject Index
pp.：
652 – 664
Color Plates
pp.：
664 – 676
Biothermodynamics, Part B
( Volume 466 )
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