Automation: Genomic and Functional Analyses :Genomic and Functional Analyses ( Volume 28 )

Publication subTitle :Genomic and Functional Analyses

Publication series :Volume 28

Author: Craig   Alister G.;Hoheisel   Jörg D.  

Publisher: Elsevier Science‎

Publication year: 1998

E-ISBN: 9780080860572

P-ISBN(Paperback): 9780125215275

P-ISBN(Hardback):  9780125215275

Subject: Q93-3 Study on microbial and microbial experiment

Language: ENG

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Description

Automation is the major future trend for many areas in microbiology, molecular biology, and biochemistry, among other disciplines. It is an enormously exciting area, where techniques and assays that were once repetitive, tedious, and time consuming can be performed robotically, liberating the time of researchers and hospital laboratory workers for more interesting work. Many techniques have now been automated and often miniaturized, including PCR analysis, DNA/RNA preparation, diagnostic test (e.g., Pap tests), compound screening, and of course, sequencing. Some major advances, notably in Professor Leroy Hood's group, have resulted in the ability to perform thousands of assays simultaneously on a normal microscope slide.

Automation, edited by two of the leading experts in the field, presents the very latest experimental techniques explained in detail. This book has succeeded in bringing together researchers at the forefront of clone library construction, genome analysis, sequencing, computational data evaluation and functional analysis, to provide insight into this "new age" of research based on genomic and chemical screening.

  • Describes automated procedures used in microbiology and molecular biology
  • Includes developments in robotics and vision systems
  • Features automation in library picking, presentation and analysis
  • Discusses paralogous duplications in microbial genomes
  • Covers deciphering genomes through automated la

Chapter

Front Cover

pp.:  1 – 4

Copyright Page

pp.:  5 – 6

Contents

pp.:  6 – 8

Contributors

pp.:  8 – 11

Foreword

pp.:  11 – 12

Introduction

pp.:  12 – 18

Chapter 1. Automation in Clinical Microbiology

pp.:  18 – 34

Chapter 2. Vision Systems for Automated Colony and Plaque Picking

pp.:  34 – 84

Chapter 3. Library Picking, Presentation and Analysis

pp.:  84 – 100

Chapter 4. The PREPSEQ Robot: An Integrated Environment for Fully Automated

pp.:  100 – 110

Chapter 5. Building Realistic Automated Production Lines for Genetic Analysis

pp.:  110 – 148

Chapter 6. Examples of Automated Genetic Analysis Developments

pp.:  148 – 172

Chapter 7. Deciphering Genomes Through Automated Large-scale Sequencing

pp.:  172 – 210

Chapter 8. DNA Arrays for Transcriptional Profiling

pp.:  210 – 222

Chapter 9. Large-scale Phenotypic Analysis in Microtitre Plates of Mutants with Deleted Open Reading Frames from Yeast Chromosome III: Key-step Between Genomic Sequencing and Protein Function

pp.:  222 – 246

Chapter 10. Automatic Analysis of Large-scale Pairwise Alignments of ProteinSequences

pp.:  246 – 262

Chapter 11. Towards Automated Prediction of Protein Function from Microbial Genomic Sequences

pp.:  262 – 282

Index

pp.:  282 – 288

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