Description
Mitosis and Meiosis details the wide variety of methods currently used to study how cells divide as yeast and insect spermatocytes, higher plants, and sea urchin zygotes. With chapters covering micromanipulation of chromosomes and making, expressing, and imaging GFP-fusion proteins, this volume contains state-of-the-art "how to" secrets that allow researchers to obtain novel information on the biology of centrosomes and kinetochores and how these organelles interact to form the spindle.
Chapters Contain Information On:
* How to generate, screen, and study mutants of mitosis in yeast, fungi, and flies
* Techniques to best image fluorescent and nonfluorescent tagged dividing cells
* The use and action of mitoclastic drugs
* How to generate antibodies to mitotic components and inject them into cells
* Methods that can also be used to obtain information on cellular processes in nondividing cells
Chapter
Chapter 2. Studying the Composition and Function of Centrosomes in Vertebrates
pp.:
30 – 52
Chapter 3. Isolation of Centrosomes from Spisulu solidissima Oocytes
pp.:
52 – 74
Chapter 4. Methods for in Situ Localization of Proteins and DNA in the Centromere–Kinetochore Complex
pp.:
74 – 98
Chapter 5. Three-Dimensional Transmission Electron Microscopy and Its Application to Mitosis Research
pp.:
98 – 130
Chapter 6. Enlightening Mitosis: Construction and Expression of Green Fluorescent Protein Fusion Proteins
pp.:
130 – 154
Chapter 7. Recombinant p50/Dynamitin as a Tool to Examine the Role of Dynactin in Intracellular Processes
pp.:
154 – 162
Chapter 8. In Vitro Assays for Studying Saccharomyces cerevisiae Kinetochore Activity
pp.:
162 – 172
Chapter 9. Fluorescent Speckle Microscopy of Spindle Microtubule Assembly and Motility in Living Cells
pp.:
172 – 192
Chapter 10. Polarized Light Microscopy of Spindles
pp.:
192 – 226
Chapter 11. Micromanipulation of Chromosomes and Spindles in Insect Spermatocytes
pp.:
226 – 236
Chapter 12. Microinjection of Mitotic Cells
pp.:
236 – 250
Chaspter 13. Obtaining Antibodies to Spindle Components
pp.:
250 – 262
Chapter 14. Using Antisense Technology to Study Mitosis
pp.:
262 – 284
Chapter 15. The Use and Action of Drugs in Analyzing Mitosis
pp.:
284 – 314
Chapter 16. Correlative Light and Electron Microscopy of Mitotic Cells in Monolayer Cultures
pp.:
314 – 334
Chapter 17. Identification and Characterization of Mitotic Mutations in Drosophila
pp.:
334 – 364
Chapter 18. Methods for Isolating and Analyzing Mitotic Mutants in Aspergillus nidulans
pp.:
364 – 386
Chapter 19. Using Green Fluorescent Protein Fusion Proteins to Quantitate Microtubule and Spindle Dynamics in Budding Yeast
pp.:
386 – 402
Chapter 20. The Use of Xenopus Egg Extracts to Study Mitotic Spindle Assembly and Function in Vitro
pp.:
402 – 430
Chapter 21. Methods for Studying Cell Division in Higher Plants
pp.:
430 – 456
Chapter 22. Using Sea Urchin Gametes for the Study of Mitosis
pp.:
456 – 490