Mitosis and Meiosis :Mitosis and Meiosis ( Volume 61 )

Publication subTitle :Mitosis and Meiosis

Publication series :Volume 61

Author: Wilson   Leslie;Matsudaira   Paul T.;Rieder   Conly L.  

Publisher: Elsevier Science‎

Publication year: 1998

E-ISBN: 9780080859590

P-ISBN(Paperback): 9780125441636

P-ISBN(Hardback):  9780125441636

Subject: Q253 Cell growth and cell division.

Language: ENG

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Description

Mitosis and Meiosis details the wide variety of methods currently used to study how cells divide as yeast and insect spermatocytes, higher plants, and sea urchin zygotes. With chapters covering micromanipulation of chromosomes and making, expressing, and imaging GFP-fusion proteins, this volume contains state-of-the-art "how to" secrets that allow researchers to obtain novel information on the biology of centrosomes and kinetochores and how these organelles interact to form the spindle.

Chapters Contain Information On:
* How to generate, screen, and study mutants of mitosis in yeast, fungi, and flies
* Techniques to best image fluorescent and nonfluorescent tagged dividing cells
* The use and action of mitoclastic drugs
* How to generate antibodies to mitotic components and inject them into cells
* Methods that can also be used to obtain information on cellular processes in nondividing cells

Chapter

Front Cover

pp.:  1 – 4

Copyright Page

pp.:  5 – 6

Contents

pp.:  6 – 12

Contributors

pp.:  12 – 16

Preface

pp.:  16 – 18

Chapter 2. Studying the Composition and Function of Centrosomes in Vertebrates

pp.:  30 – 52

Chapter 3. Isolation of Centrosomes from Spisulu solidissima Oocytes

pp.:  52 – 74

Chapter 4. Methods for in Situ Localization of Proteins and DNA in the Centromere–Kinetochore Complex

pp.:  74 – 98

Chapter 5. Three-Dimensional Transmission Electron Microscopy and Its Application to Mitosis Research

pp.:  98 – 130

Chapter 6. Enlightening Mitosis: Construction and Expression of Green Fluorescent Protein Fusion Proteins

pp.:  130 – 154

Chapter 7. Recombinant p50/Dynamitin as a Tool to Examine the Role of Dynactin in Intracellular Processes

pp.:  154 – 162

Chapter 8. In Vitro Assays for Studying Saccharomyces cerevisiae Kinetochore Activity

pp.:  162 – 172

Chapter 9. Fluorescent Speckle Microscopy of Spindle Microtubule Assembly and Motility in Living Cells

pp.:  172 – 192

Chapter 10. Polarized Light Microscopy of Spindles

pp.:  192 – 226

Chapter 11. Micromanipulation of Chromosomes and Spindles in Insect Spermatocytes

pp.:  226 – 236

Chapter 12. Microinjection of Mitotic Cells

pp.:  236 – 250

Chaspter 13. Obtaining Antibodies to Spindle Components

pp.:  250 – 262

Chapter 14. Using Antisense Technology to Study Mitosis

pp.:  262 – 284

Chapter 15. The Use and Action of Drugs in Analyzing Mitosis

pp.:  284 – 314

Chapter 16. Correlative Light and Electron Microscopy of Mitotic Cells in Monolayer Cultures

pp.:  314 – 334

Chapter 17. Identification and Characterization of Mitotic Mutations in Drosophila

pp.:  334 – 364

Chapter 18. Methods for Isolating and Analyzing Mitotic Mutants in Aspergillus nidulans

pp.:  364 – 386

Chapter 19. Using Green Fluorescent Protein Fusion Proteins to Quantitate Microtubule and Spindle Dynamics in Budding Yeast

pp.:  386 – 402

Chapter 20. The Use of Xenopus Egg Extracts to Study Mitotic Spindle Assembly and Function in Vitro

pp.:  402 – 430

Chapter 21. Methods for Studying Cell Division in Higher Plants

pp.:  430 – 456

Chapter 22. Using Sea Urchin Gametes for the Study of Mitosis

pp.:  456 – 490

Index

pp.:  490 – 508

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