Cilia and Flagella ( Volume 47 )

Publication series :Volume 47

Author: Wilson   Leslie;Matsudaira   Paul T.;Dentler   William  

Publisher: Elsevier Science‎

Publication year: 1995

E-ISBN: 9780080859453

P-ISBN(Paperback): 9780125641487

P-ISBN(Hardback):  9780125641487

Subject: Q25 Cell Physiol.

Language: ENG

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Description

Cilia and Flagella presents protocols accessible to all individuals working with eukaryotic cilia and flagella. These recipes delineate laboratory methods and reagents, as well as critical steps and pitfalls of the procedures. The volume covers the roles of cilia and flagella in cell assembly and motility, the cell cycle, cell-cell recognition and other sensory functions, as well as human diseases and disorders. Students, researchers, professors, and clinicians should find the book's combination of "classic" and innovative techniques essential to the study of cilia and flagella.

Key Features
* A complete guide containing more than 80 concise technical chapters friendly to both the novice and experienced researcher
* Covers protocols for cilia and flagella across systems and species from Chlamydomonas and Euglena to mammals
* Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time, including microscopy, electrophoresis, and PCR
* Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes, who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures

Chapter

Front Cover

pp.:  1 – 4

Copyright Page

pp.:  5 – 8

Contents

pp.:  8 – 26

Contributors

pp.:  26 – 32

Preface

pp.:  32 – 34

Introduction

pp.:  34 – 38

Part I: Isolation of Cilia and Flagella

pp.:  38 – 92

Part II: Culture and Isolation of Ciliated Epithelial Cells and Cilia

pp.:  92 – 136

Part III: General Methods

pp.:  136 – 188

Part IV: Structure

pp.:  188 – 244

Part V: Motors and Motion Analysis

pp.:  244 – 338

Part VI: Cytoskeletal Preparations

pp.:  338 – 392

Part VII: Flagellar Fractionation and Membrane Characterization

pp.:  392 – 484

Part VIII: Assay and Isolation of Flagellar Enzymes

pp.:  484 – 550

Part IX: Genetics and Molecular Tools for the Analysis of Cilia and Flagella

pp.:  550 – 626

Index

pp.:  626 – 638

Volumes in Series

pp.:  638 – 644

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