Description
Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.
Key Features
* Completely revised and greatly expanded since the publication of the First Edition in 1990
* Methods cover cell death and cell cycle analyses
Practical, handbook-style presentation works in lab or classroom
* Unique comprehensive methodological coverage
* Color plates illustrate techniques
* In-depth treatment of procedures, including a description of each procedure:
* Theoretical foundations
* Critical aspects
* Possible pitfalls
* Written by authors with extensive experience who:
* Developed or modified the techniques
* Describe their experience with different instruments and applications to different cell systems
* Are the Who's Who in Flow Cytometry
Chapter
Chapter 1. Dissociation of Intact Cells from Tumors and Normal Tissues
pp.：
34 – 48
Chapter 2. Assays of Cell Viability: Discrimination of Cells Dying by Apoptosis
pp.：
48 – 72
Chapter 3. Cell Preparation for the Identification of Leukocytes
pp.：
72 – 98
Chapter 4. Multiparameter Analysis of Leukocytes by Flow Cytometry
pp.：
98 – 120
Chapter 5. Immunophenotyping Using Fixed Cells
pp.：
120 – 134
Chapter 6. Simultaneous DNA Content and Cell-Surface Immunofluorescence Analysis
pp.：
134 – 142
Chapter 7. Flow Cytometry Crossmatching for Solid Organ Transplantation
pp.：
142 – 160
Chapter 8. Cell Membrane Potential Analysis
pp.：
160 – 174
Chapter 9. Measurement of Intracellular pH
pp.：
174 – 188
Chapter 10. Intracellular Ionized Calcium
pp.：
188 – 214
Chapter 11. Cellular Protein Content Measurements
pp.：
214 – 224
Chapter 12. Lysosomal Proton Pump Activity: Supravital Cell Staining with Acridine Orange Differentiates Leukocyte Subpopulations
pp.：
224 – 234
Chapter 13. Staining of DNA in Live and Fixed Cells
pp.：
234 – 250
Chapter 14. High-Resolution Analysis of Nuclear DNA Employing the Fluorochrome DAPI
pp.：
250 – 258
Chapter 15. Detergent and Proteolytic Enzyme-Based Techniques for Nuclear Isolation and DNA Content Analysis
pp.：
258 – 270
Chapter 16. DNA Analysis from Paraffin-Embedded Blocks
pp.：
270 – 280
Chapter 17. Controls, Standards, and Histogram Interpretation in DNA Flow Cytometry
pp.：
280 – 302
Chapter 18. DNA Content Histogram and Cell-Cycle Analysis
pp.：
302 – 336
Chapter 19. Imniunochemical Quantitation of Bromodeoxyuridine: Application to Cell-Cycle Kinetics
pp.：
336 – 356
Chapter 20. Application and Detection of IdUrd and CldUrd as Two Independent Cell-Cycle Markers
pp.：
356 – 366
Chapter 21. Cell-Cycle Analysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33358–Ethidium Bromide Bivariate Flow Cytometry
pp.：
366 – 380
Chapter 22. Detection of BrdUrd-Labeled Cells by Differential Fluorescence Analysis of DNA Fluorochromes: Pulse-Chase and Continuous Labeling Methods
pp.：
380 – 390
Chapter 23. Analysis of Intracellular Proteins
pp.：
390 – 416
Chapter 24. “Washless” Procedures for Nuclear Antigen Detection
pp.：
416 – 428
Chapter 25. Light Scatter of Isolated Cell Nuclei as a Parameter Discriminating the Cell-Cycle Subcompartments
pp.：
428 – 440
Chapter 26. Simultaneous Analysis of Cellular RNA and DNA Content
pp.：
440 – 460
Chapter 27. Analysis of DNA Content and Cyclin Protein Expression in Studies of DNA Ploidy, Growth Fraction, Lymphocyte Stimulation, and the Cell Cycle
pp.：
460 – 476
Chapter 28. Oxidative Product Formation Analysis by Flow Cytometry
pp.：
476 – 488
Chapter 29. Flow Cytometric Determination of Cysteine and Serine Proteinase Activities in Living Cells with Rhodamine 110 Substrates
pp.：
488 – 500
Chapter 30. Leucine Aminopeptidase Activity by Flow Cytometry
pp.：
500 – 508
Chapter 31. Enzyme Kinetics
pp.：
508 – 548
Chapter 32. On-Line Flow Cytometry: A Versatile Method for Kinetic Measurements
pp.：
548 – 566
Chapter 33. Acid-Induced Denaturation of DNA in Situ as a Probe of Chromatin Structure
pp.：
566 – 582
Chapter 34. Detection of Intracellular Virus and Viral Products
pp.：
582 – 598
Volumes in Series
pp.：
626 – 634
Flow Cytometry, Part A
( 2 )
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