Front Cover

pp.：  1 – 6

Methods in Cell Biology, Volume 30

pp.：  6 – 7

Copyright Page

pp.：  7 – 8

Contents

pp.：  8 – 16

Contributors

pp.：  14 – 18

Preface

pp.：  16 – 14

Chapter 1. Image Fidelity: Characterizing the Imaging Tranfer Function

pp.：  18 – 64

Chapter 2. Validation of an Imaging System

pp.：  64 – 102

Chapter 3. Imaging of Unresolved Objects, Superresolution, and Precision of Distance Measurement, with Video Microscopy

pp.：  102 – 130

Chapter 4. Fluorescent Standards

pp.：  130 – 144

Chapter 5. Fluorescent Indicators of Ion Concentrations

pp.：  144 – 174

Chapter 6. Fluorescence Ratio Imaging Microscopy

pp.：  174 – 210

Chapter 7. Fluorescent Indicators of Membrane Potential: Microspectrofluorometry and Imaging

pp.：  210 – 236

Chapter 8. Resonance Energy Transfer Microscopy

pp.：  236 – 262

Chapter 9. Total Internal Reflection Fluorescence Microscopy

pp.：  262 – 288

Chapter 10. Designing, Building, and Using a Fluorescence Recovery after Photobleaching Instrument

pp.：  288 – 324

Chapter 11. Interpretation of Fluorescence Correlation Spectroscopy and Photobleaching Recovery in Terms of Molecular Interactions

pp.：  324 – 350

Chapter 12. Fluorescence Polarization Microscopy

pp.：  350 – 370

Chapter 13. Fluorescence Microscopy in Three Dimensions

pp.：  370 – 398

Chapter 14. Three Dimensional Confocal Fluorescence Microscopy

pp.：  398 – 418

Chapter 15. Emission of Fluorescence at an Interface

pp.：  418 – 436

Chapter 16. Fluorescence Labeling and Microscopy of DNA

pp.：  436 – 468

Chapter 17. Multiple Spectral Parameter Imaging

pp.：  468 – 498

Index

pp.：  498 – 518

Content of Resent Volumes

pp.：  518 – 524