The Cytoskeleton, Part A :Cytoskeleton Proteins, Isolation and Characterization ( Volume 24 )

Publication subTitle :Cytoskeleton Proteins, Isolation and Characterization

Publication series :Volume 24

Author: Wilson   Leslie  

Publisher: Elsevier Science‎

Publication year: 1982

E-ISBN: 9780080859224

P-ISBN(Paperback): 9780125641241

P-ISBN(Hardback):  9780125641241

Subject: Q28 cell biology

Language: ENG

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Description

The Cytoskeleton, Part A

Chapter

Front Cover

pp.:  1 – 4

Copyright Page

pp.:  5 – 6

Contents

pp.:  6 – 14

Contributors

pp.:  14 – 16

Preface

pp.:  16 – 18

Chapter 2. Purification and Chemical Properties of Brain Tubulin

pp.:  26 – 48

Chapter 3. Assembly –Disassembly Purification and Characterization of Microtubule Protein without Glycerol

pp.:  48 – 68

Chapter 4. A Brain Microtubule Protein Preparation Depleted of Mitochondrial and Synaptosomal Components

pp.:  68 – 78

Chapter 5. Purification and Reassembly of Tubulin from Outer Doublet Microtubules

pp.:  78 – 96

Chapter 6. Production of Antisera and Radioimmunoassays for Tubulin

pp.:  96 – 114

Chapter 7. Immunofluorescence and Immunocytochemical Procedures with Affinity Purified Antibodies: Tubulin-Containing Structures

pp.:  114 – 150

Chapter 8. An Automated Method for Defining Microtubule Length Distributions

pp.:  150 – 162

Chapter 9. Measurement of Steady-State Tubulin Flux

pp.:  162 – 176

Chapter 10. A Rapid Filtration Assay for Analysis of Microtubule Assembly, Disassembly, and Steady-State Tubulin Flux

pp.:  176 – 188

Chapter 11. A Direct Method for Analyzing the Polymerization Kinetics at the Two Ends of a Microtubule

pp.:  188 – 206

Chapter 12. Dynein Decoration of Microtubules—Determination of Polarity

pp.:  206 – 224

Chapter 13. Microtubule Polarity Determination Based on Conditions for Tubulin Assembly in Vitro

pp.:  224 – 234

Chapter 14. The Use of Tannic Acid in Microtubule Research

pp.:  234 – 252

Chapter 15. The Cyclic Tyrosination/Detyrosination of Alpha Tubulin

pp.:  252 – 274

Chapter 16. Tubulin –Tyrosine Ligase from Brain

pp.:  274 – 282

Chapter 17. Preparation of Brain Tyrosinotubulin Carboxypeptidase

pp.:  282 – 288

Chapter 18. Purifcution of Muscle Actin

pp.:  288 – 308

Chapter 19. Directional Growth of Actin Off Polylysine-Coated Beads

pp.:  308 – 318

Chapter 20. A Falling Ball Apparatus to Measure Filament Cross-Linking

pp.:  318 – 330

Chapter 21. Antibody Production and Immunofluorescent Characterization of Actin and Contractile Proteins

pp.:  330 – 350

Chapter 22. Myosin Purification and Characterization

pp.:  350 – 390

Chapter 23. Preparation and Purification of Dynein

pp.:  390 – 416

Chapter 24. Isolation and Characterization of Intermediate Filaments

pp.:  416 – 438

Index

pp.:  438 – 446

Contents of Recent Volumes

pp.:  446 – 464

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