Ultrasensitive and Rapid Enzyme Immunoassay ( Volume 27 )

Publication series :Volume 27

Author: Ishikawa   E.  

Publisher: Elsevier Science‎

Publication year: 1999

E-ISBN: 9780080858968

P-ISBN(Paperback): 9780444502018

P-ISBN(Hardback):  9780444502018

Subject: R446.61 Immunological test method

Language: ENG

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Description

Immunoassay techniques have become essential in various fields of pure and applied research. This volume of the well known Laboratory Techniques series will be of assistance to those who have plans or are making efforts to develop ultrasensitive enzyme immunoassays for antigens and antibodies.

The volume describes factors limiting the sensitivity of noncompetitive solid phase enzyme immunoassays, methods to overcome difficulties limiting sensitivities, methods to perform ultrasensitive enzyme immunoassays as rapidly as possible, and protocols of enzyme labeling and enzyme assays as well as ultrasensitive enzyme immunoassays.

Ultrasensitive and Rapid Enzyme Immunoassay, with its clear presentation and up-to-date information, will be essential to scientists in pharmaceutical companies and in applied research.

Chapter

Front Cover

pp.:  1 – 4

Copyright Page

pp.:  5 – 8

Preface

pp.:  6 – 18

Contents

pp.:  8 – 6

Chapter 2. History of ultrasensitive enzyme immunoassays

pp.:  22 – 24

Chapter 3. Factors limiting the sensitivity of noncompetitive heterogeneous solid phase enzyme immunoassays

pp.:  24 – 34

Chapter 4. Methods to minimize effects of factors limiting the sensitivity

pp.:  34 – 96

Chapter 5. Principle of the immune complex transfer enzyme immunoassay

pp.:  96 – 114

Chapter 6. Potential of the immune complex transfer enzyme immunoassay to improve the sensitivity and its limitations

pp.:  114 – 158

Chapter 7. Ultrasensitive and rapid enzyme immunoassay

pp.:  158 – 194

Chapter 8. Protocol of immune complex transfer enzyme immunoassay

pp.:  194 – 210

Chapter 9. Ultrasensitive immunoassay for haptens

pp.:  210 – 228

Chapter 10. Properties of the reagents for enzyme-labeling

pp.:  228 – 240

Chapter 11. Enzyme-labeling of antibodies

pp.:  240 – 266

Chapter 12. Preparation and affinity-purification of IgG fragments

pp.:  266 – 278

Chapter 13. Protocol for enzyme-labeling of antibodies

pp.:  278 – 294

Chapter 14. Protocol for labeling avidin with 2,4-dinitrophenyl groups and β-D-galactosidase

pp.:  294 – 300

Chapter 15. Enzyme-labeling of antigens

pp.:  300 – 306

Chapter 16. Protocol for enzyme-labeling of antigens

pp.:  306 – 320

Chapter 17. Assays of enzymes

pp.:  320 – 328

References

pp.:  328 – 344

Subject Index

pp.:  344 – 352

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