Methods of Cell Separation ( Volume 18 )

Publication series :Volume 18

Author: Sharpe   P. T.  

Publisher: Elsevier Science‎

Publication year: 1988

E-ISBN: 9780080858876

P-ISBN(Paperback): 9780444809308

P-ISBN(Hardback):  9780444809308

Subject: Q6 Biophysics

Language: ENG

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Description

Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments.

A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for each method.

Methods of Cell Separation is distinguished by three powerful assets: descriptions of the majority of cell separation methods currently being used; details of the experimental procedures involved in each method; and comparisons of the different methods for separating cell populations with particular reference to blood lymphocytes.

An excellent addition to a distinguished series, and extremely useful as a laboratory manual.

Chapter

Front Cover

pp.:  1 – 4

Methods of Cell Separation

pp.:  4 – 5

Copyright Page

pp.:  5 – 8

Abbreviations

pp.:  6 – 16

Contents

pp.:  8 – 6

Chapter 1. Introduction

pp.:  16 – 22

Chapter 2. Methods of cell counting and assaying cell viability

pp.:  22 – 33

Chapter 3. Centrifugation

pp.:  33 – 85

Chapter 4. Unit gravity sedimentation

pp.:  85 – 106

Chapter 5. Centrifugal elutriation

pp.:  106 – 122

Chapter 6. Aqueous two-phase partition

pp.:  122 – 158

Chapter 7. Electrophoresis

pp.:  158 – 172

Specific surface methods – an introduction to Chapters 8–13

pp.:  172 – 174

Chapter 8. Affinity chromatography

pp.:  174 – 202

Chapter 9. Affinity separation on fibres and surfaces

pp.:  202 – 215

Chapter 10. Affinity partition

pp.:  215 – 223

Chapter 11. Flow sorting

pp.:  223 – 236

Chapter 12. Magnetic cell sorting

pp.:  236 – 253

Chapter 13. Separation of cells in different phases of the cell cycle

pp.:  253 – 276

Appendix

pp.:  276 – 279

References

pp.:  279 – 284

General references for further reading

pp.:  284 – 285

Subject index

pp.:  285 – 288

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